JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB312362

重组Alexa Fluor® 568荧光Anti-APC抗体[EP701Y]

Alexa Fluor® 568 Anti-APC antibody [EP701Y]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal APC antibody - conjugated to Alexa Fluor® 568.

查看别名

DP2.5, APC, Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 2.5

不同偶联物与剂型 (10)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP701Y

亚型

IgG

偶联物

Alexa Fluor® 568

激发波长/发射波长

Ex: 578nm, Em: 603nm

不含载体蛋白

No

应用

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody is predicted to detect isoform 2 (short) of APC based on sequence analysis.

style
left-column
style
left-column

产品详情

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

APC or Adenomatous Polyposis Coli is a multifunctional protein involved in several cellular processes. Its molecular weight is approximately 310 kDa. The APC protein is expressed in various tissues with higher concentrations found particularly in the colon and intestinal epithelial cells. Mechanically APC acts as a scaffold protein interacting with components of the cytoskeleton and regulating processes such as cell migration and adhesion. Its ability to bind to microtubules is key in its role in stabilizing them which affects cellular polarity and division.
Biological function summary

APC plays a significant role in the regulation of the Wnt signaling pathway a critical mediator of cell growth and differentiation. APC forms part of a larger destruction complex that includes proteins like Axin and GSK-3β. This complex degrades β-catenin preventing its accumulation in the cell nucleus and therefore modulating gene transcription involved in cell proliferation. The ability of APC to mediate this process is important for maintaining proper cellular homeostasis and preventing uncontrolled cell growth that may lead to neoplasms.

Pathways

APC is integral to the Wnt signaling and PI3K/Akt pathways. The Wnt pathway primarily concerns the regulation of cellular growth with APC facilitating the degradation of β-catenin therefore inhibiting the transcription of proliferative genes. Meanwhile in the PI3K/Akt pathway interactions between APC and β-catenin may influence cell survival and apoptosis. This connectivity to PI3K and Wnt pathways highlights APC's role in both promoting and inhibiting cell division processes depending on cellular context and signaling input.

APC is intrinsically linked to colorectal cancer and familial adenomatous polyposis (FAP). Mutations or loss of function in the APC gene lead to unregulated β-catenin levels promoting tumorigenesis in the colon. In colorectal cancer the absence or dysfunction of APC disrupts normal central pathways resulting in proliferative advantage to mutated cells. Connections to β-catenin further emphasize the mechanistic role APC plays in predisposing individuals to these diseases where abnormal Wnt pathway signaling becomes deleterious. Additionally disruption in APC function and the related pathways can alter interactions with other proteins like Axin and GSK-3β which are often involved in these disorders.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton (PubMed : 17293347). Plays a role in mediating the organization of F-actin into ordered bundles (PubMed : 17293347). Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules (By similarity). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
See full target information APC
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Version
Primary Antibodies

AB239828

Anti-APC antibody [EP701Y] - BSA and Azide free

primary-antibodies

apc-antibody-ep701y-bsa-and-azide-free-ab239828

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com