AB207014

重组Alexa Fluor® 555荧光Anti-STAT6抗体[YE361]

Alexa Fluor® 555 Anti-STAT6 antibody [YE361]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal STAT6 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

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Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

ab207014 staining STAT6 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207014 at a 1/100 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor^® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

ab207014 staining STAT6 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207014 at a 1/100 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor^® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

不同偶联物与剂型 (9)

Carrier free

Anti-STAT6 antibody [YE361] - BSA and Azide free
Unconjugated

Anti-STAT6 antibody [YE361]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-STAT6 antibody [YE361]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-STAT6 antibody [YE361]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-STAT6 antibody [YE361]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-STAT6 antibody [YE361]
519 FITC

FITC Anti-STAT6 antibody [YE361]
HRP

HRP Anti-STAT6 antibody [YE361]
578 PE

PE Anti-STAT6 antibody [YE361]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

YE361

亚型

IgG

偶联物

Alexa Fluor® 555

激发波长/发射波长

Ex: 555nm, Em: 565nm

不含载体蛋白

反应种属

Human, Human, Mouse

应用

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

See full target information STAT6

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102154 PubMed38511173

2024

STAT6-targeting antisense oligonucleotides against solitary fibrous tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Jose L Mondaza-Hernandez,David S Moura,Alexey S Revenko,Angelica Tolentino,John T Nguyen,Nam Tran,Clark A Meyer,Jose Merino-Garcia,Rafael Ramos,Davide Di Lernia,Javier Martin-Broto,Heather N Hayenga,Leonidas Bleris

Nature communications 14:7479 PubMed37980418

2023

Comprehensive analysis reveals potential therapeutic targets and an integrated risk stratification model for solitary fibrous tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Renjing Zhang,Yang Yang,Chunfang Hu,Mayan Huang,Wenjian Cen,Dongyi Ling,Yakang Long,Xin-Hua Yang,Boheng Xu,Junling Peng,Sujie Wang,Weijie Zhu,Mingbiao Wei,Jiaojiao Yang,Yuxia Xu,Xu Zhang,Jiangjun Ma,Fang Wang,Hongtu Zhang,Peiqing Ma,Xiaojun Zhu,Guohui Song,Li-Yue Sun,De-Shen Wang,Feng-Hua Wang,Yu-Hong Li,Sandro Santagata,Qin Li,Yan-Fen Feng,Ziming Du

View all publications

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重组Alexa Fluor® 555荧光Anti-STAT6抗体[YE361]