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AB319846

重组Alexa Fluor® 555荧光Anti-PFKM + PFKL抗体[EPR11904]

Alexa Fluor® 555 Anti-PFKM + PFKL antibody [EPR11904]

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Rabbit Recombinant Monoclonal PFKM antibody - conjugated to Alexa Fluor® 555.

查看别名

PFKX, PFKM, ATP-PFK, PFK-M, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, Phosphohexokinase, PFK-A

不同偶联物与剂型 (5)

  • 660 APC

    APC Anti-PFKM + PFKL antibody [EPR11904]

  • 578 PE

    PE Anti-PFKM + PFKL antibody [EPR11904]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PFKM + PFKL antibody [EPR11904]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PFKM + PFKL antibody [EPR11904]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PFKM + PFKL antibody [EPR11904]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11904

亚型

IgG

偶联物

Alexa Fluor® 555

激发波长/发射波长

Ex: 555nm, Em: 565nm

不含载体蛋白

No

应用

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PFKM (phosphofructokinase muscle) and PFKL (phosphofructokinase liver) are key enzymes in glycolysis. PFKM is also known as phosphofructokinase-1 muscle type and has a mass of approximately 85 kDa while PFKL also has a similar mass. These enzymes are expressed mainly in their respective tissues—PFKM in muscle and PFKL in the liver—where they catalyze the conversion of fructose-6-phosphate to fructose-16-bisphosphate. This reaction is important for committing glucose to the glycolytic pathway controlling the rate of glycolysis.
Biological function summary

PFKM and PFKL play significant roles in cellular metabolism. They form homotetramers or heterotetramers in the presence of PFKP (phosphofructokinase platelet) another isozyme. The tetrameric enzyme complexes function as a significant control point for the glycolytic flux responding to allosteric activators like AMP and inhibitors such as ATP and citrate therefore regulating ATP production based on cellular energy demands.

Pathways

PFKM and PFKL are integral to glycolysis one of the primary metabolic pathways for energy production. They are involved in the regulation of energy homeostasis by interacting with other glycolytic enzymes such as hexokinase and pyruvate kinase. Their action ensures efficient conversion of glucose to pyruvate linking to the citric acid cycle and oxidative phosphorylation within aerobic respiration.

PFKM and PFKL have connections to glycogen storage disease type VII (Tarui's disease) and cancer metabolism. In Tarui's disease mutations in the PFKM gene impair glycolysis in muscle tissues leading to exercise-induced muscle weakness. Furthermore cancer cells often exhibit altered PFKL expression attributed to their increased glycolytic rate for rapid proliferation. The interplay between these enzymes and regulation by p53 which can modulate PFKL activity influences glycolytic pathways in cancerous tissues.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
See full target information PFKM

其他靶点

PFKL

Abcam Product Promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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