AB307126

重组Alexa Fluor® 555荧光Anti-Periostin抗体[EPR20806]

Alexa Fluor® 555 Anti-Periostin antibody [EPR20806]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Periostin antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

查看别名

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Periostin antibody [EPR20806] (AB307126)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Periostin antibody [EPR20806] (AB307126)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Periostin with ab307126 at 1/100 (5.0 ug/ml). Positive staining on human stomach. The section was incubated with ab307126 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Periostin antibody [EPR20806] (AB307126)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Periostin with ab307126 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on mouse stomach. The section was incubated with ab307126 at 4ЎгC overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount?.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Periostin antibody [EPR20806] (AB307126)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Periostin with ab307126 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on rat stomach. The section was incubated with ab307126 at 4ЎгC overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount?.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

不同偶联物与剂型 (3)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Periostin antibody [EPR20806]
Unconjugated

Anti-Periostin antibody [EPR20806]
Carrier free

Anti-Periostin antibody [EPR20806] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20806

亚型

IgG

偶联物

Alexa Fluor® 555

激发波长/发射波长

Ex: 555nm, Em: 565nm

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.

Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

See full target information POSTN

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Alexa Fluor® 555荧光Anti-Periostin抗体[EPR20806]