重组Alexa Fluor® 555荧光Anti-Dysferlin抗体[JAI-1-49-3] (ab317597)
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Dysferlin antibody [JAI-1-49-3] (ab317597)](https://www.abcam.cn/ps/products/317/ab317597/Images/ab317597-2-Anti-Dysferlin-antibody-JAI-1-49-3-A555-immunohistochemistry-skeletal-muscle-mouse.jpg "Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Dysferlin antibody [JAI-1-49-3] (ab317597)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [JAI-1-49-3] to Dysferlin
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 555荧光Anti-Dysferlin抗体[JAI-1-49-3]
参阅全部 Dysferlin 一抗 -
描述
Alexa Fluor® 555荧光兔单克隆抗体[JAI-1-49-3] to Dysferlin -
宿主
Rabbit -
偶联物
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC-Fr: Frozen mouse normal skeletal muscle.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
JAI-1-49-3 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
- Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)
- Anti-Dysferlin antibody [JAI-1-49-3] - Low endotoxin, Azide free (ab215376)
- Alexa Fluor® 594 Anti-Dysferlin antibody [JAI-1-49-3] (ab311738)
- Alexa Fluor® 568 Anti-Dysferlin antibody [JAI-1-49-3] (ab313017)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317597于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
1/50.
|
| 说明 |
|---|
|
IHC-Fr
1/50. |
靶标
-
功能
Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. -
组织特异性
Expressed in skeletal muscle, myoblast, myotube and in the syncytiotrophoblast (STB) of the placenta (at protein level). Highly expressed in skeletal muscle. Also found in heart, brain, spleen, intestine, placenta and at lower levels in liver, lung, kidney and pancreas. -
疾病相关
Defects in DYSF are the cause of limb-girdle muscular dystrophy type 2B (LGMD2B) [MIM:253601]. LGMD2B is an autosomal recessive degenerative myopathy characterized by weakness and atrophy starting in the proximal pelvifemoral muscles, with onset in the late teens or later, massive elevation of serum creatine kinase levels and slow progression. Scapular muscle involvement is minor and not present at onset. Upper limb girdle involvement follows some years after the onset in lower limbs.
Defects in DYSF are the cause of Miyoshi muscular dystrophy type (MMD1) [MIM:254130]. MMD1 is a late-onset muscular dystrophy involving the distal lower limb musculature. It is characterized by weakness that initially affects the gastrocnemius muscle during early adulthood. Otherwise the phenotype overlaps with LGMD2B, especially in age at onset and creatine kinase elevation.
Defects in DYSF are the cause of distal myopathy with anterior tibial onset (DMAT) [MIM:606768]. Onset of the disorder is between 14 and 28 years of age and the anterior tibial muscles are the first muscle group to be involved. Inheritance is autosomal recessive. -
序列相似性
Belongs to the ferlin family.
Contains 5 C2 domains. -
发展阶段
Expression in limb tissue from 5-6 weeks embryos; persists throughout development. -
结构域
The C2 domain 1 associates with lipid membranes in a calcium-dependent manner. -
细胞定位
Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Colocalizes, during muscle differentiation, with BIN1 in the T-tubule system of myotubules and at the site of contact between two myotubes or a myoblast and a myotube. Wounding of myotubes led to its focal enrichment to the site of injury and to its relocalization in a Ca(2+)-dependent manner toward the plasma membrane. Colocalizes with AHNAK, AHNAK2 and PARVB at the sarcolemma of skeletal muscle. Detected on the apical plasma membrane of the syncytiotrophoblast. Reaches the plasmma membrane through a caveolin-independent mechanism. Retained by caveolin at the plasmma membrane (By similarity). Colocalizes, during muscle differentiation, with CACNA1S in the T-tubule system of myotubules (By similarity). Accumulates and colocalizes with fusion vesicles at the sarcolemma disruption sites. - Information by UniProt
-
数据库链接
- Entrez Gene: 26903 Mouse
- SwissProt: Q9ESD7 Mouse
- Unigene: 220982 Mouse
-
别名
- DMAT antibody
- DYSF antibody
- DYSF_HUMAN antibody
see all
图片
-
Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Dysferlin antibody [JAI-1-49-3] (ab317597)
Immunofluorescence staining of Dysferlin in a section of frozen mouse normal skeletal muscle.
The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BONDTM. The section was incubated at room temperature for 1 hour with ab317597 at 1/50 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
-
![Alexa Fluor® 555 Anti-Dysferlin antibody [JAI-1-49-3] (ab317597)](https://www.abcam.cn/ps/products/317/ab317597/Images/ab317597-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 555 Anti-Dysferlin antibody [JAI-1-49-3] (ab317597)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab317597 尚未被引用在任何文献中。