Alexa Fluor® 555荧光Anti-CNPase抗体[11-5B] (ab317592)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)](https://www.abcam.cn/ps/products/317/ab317592/Images/ab317592-2-Anti-CNPase-antibody-115B-A555-immunohistochemistry-brain-mouse.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)")
Key features and details
- Alexa Fluor® 555 Mouse monoclonal [11-5B] to CNPase
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 555荧光Anti-CNPase抗体[11-5B]
参阅全部 CNPase 一抗 -
描述
Alexa Fluor® 555荧光小鼠单克隆抗体[11-5B] to CNPase -
宿主
Mouse -
偶联物
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Mouse brain. ICC/IF: primary hippocampal rat neurons/glia.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
11-5B -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- HRP Anti-CNPase antibody [11-5B] (ab201678)
- Alexa Fluor® 647 Anti-CNPase antibody [11-5B] (ab201679)
- Anti-CNPase antibody [11-5B] - BSA and Azide free (ab237961)
- Alexa Fluor® 568 Anti-CNPase antibody [11-5B] (ab313959)
- Alexa Fluor® 488 Anti-CNPase antibody [11-5B] (ab313960)
- Alexa Fluor® 594 Anti-CNPase antibody [11-5B] (ab320064)
- Anti-CNPase antibody [11-5B] (ab6319)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317592于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. -
细胞定位
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 12799 Mouse
- Entrez Gene: 25275 Rat
- SwissProt: P16330 Mouse
- SwissProt: P13233 Rat
- Unigene: 15711 Mouse
- Unigene: 31762 Rat
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别名
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)
Immunofluorescence staining of CNPase staining in a section of formalin-fixed paraffin-embedded mouse brain normal*.
Performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 100°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab317592 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)](https://www.abcam.cn/ps/products/317/ab317592/Images/ab317592-3-Anti-CNPase-antibody-115B-A555-immunohistochemistry-skeletal-muscle-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)Negative immunofluorescence staining of CNPase in a section of formalin-fixed paraffin-embedded mouse skeletal muscle*.
Performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab317592 at 1/100 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)](https://www.abcam.cn/ps/products/317/ab317592/Images/ab317592-4-ab317592-RtNeu-STN407897-1001079605-5-ugmL-PFA.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)ab317592 staining CNPase in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab317592 at 5 μg/ml (shown in green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in magenta) was used at 2 μg/ml for structural counterstaining.
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
This product also work with 100% methanol (5 min) fixation under the same testing conditions.
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![Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)](https://www.abcam.cn/ps/products/317/ab317592/Images/ab317592-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 555 Anti-CNPase antibody [11-5B] (ab317592)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317592 尚未被引用在任何文献中。