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AB314254

重组Alexa Fluor® 555荧光Anti-ALDH1L1抗体[EPR12743(B)]

Alexa Fluor® 555 Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker

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Rabbit Recombinant Monoclonal ALDH1L1 antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

查看别名

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (AB314254)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (AB314254)

Negative immunofluorescence staining of ALDH1L1 in a section of frozen human normal skin*. The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND. The section was incubated at room temperature for 1 hour with ab314254 at 1/50 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (AB314254)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (AB314254)

Immunofluorescence staining of ALDH1L1 in a section of frozen human normal liver*. The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND. The section was incubated at room temperature for 1 hour with ab314254 at 1/50 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

不同偶联物与剂型 (6)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker

  • Unconjugated

    Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker

  • Carrier free

    Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12743(B)

亚型

IgG

偶联物

Alexa Fluor® 555

激发波长/发射波长

Ex: 555nm, Em: 565nm

不含载体蛋白

No

反应种属

Human, Human, Mouse, Rat

应用

IHC-P, IHC-Fr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.
Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed : 19933275, PubMed : 21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
See full target information ALDH1L1
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搭配使用产品

Assay Kits

AB239705

Dihydrofolate Reductase Assay Kit (Colorimetric)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

  • 0
  • 0

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