Alexa Fluor® 488荧光Anti-Tubulin抗体[YOL1/34] - Microtubule Marker (ab195883)
Key features and details
- Alexa Fluor® 488 Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 488荧光Anti-Tubulin抗体[YOL1/34] - Microtubule Marker
参阅全部 Tubulin 一抗 -
描述
Alexa Fluor® 488荧光大鼠单克隆抗体[YOL1/34] to Tubulin - Microtubule Marker -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator -
免疫原
Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.
-
阳性对照
- ICC/IF: HeLa cells.
-
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
YOL1/34 -
同种型
IgG2a -
研究领域
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab195883于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
说明 |
---|
ICC/IF
1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
靶标
-
功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
-
数据库链接
- Entrez Gene: 10376 Human
- Entrez Gene: 203068 Human
- Entrez Gene: 7280 Human
- Entrez Gene: 22143 Mouse
- Entrez Gene: 22151 Mouse
- Entrez Gene: 22153 Mouse
- Entrez Gene: 500929 Rat
- Omim: 191130 Human
see all -
别名
- Tubulin beta 2b antibody
- Alpha tubulin antibody
- Alpha-tubulin ubiquitous antibody
see all
图片
-
ab195883 staining tubulin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195883 at a 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab195883 被引用在 6 文献中.
- Kölln LS et al. Label2label: training a neural network to selectively restore cellular structures in fluorescence microscopy. J Cell Sci 135:N/A (2022). PubMed: 35022745
- Tang J et al. Two Novel Pathogenic Variants of TJP2 Gene and the Underlying Molecular Mechanisms in Progressive Familial Intrahepatic Cholestasis Type 4 Patients. Front Cell Dev Biol 9:661599 (2021). PubMed: 34504838
- Jin W et al. Theabrownin inhibits the cytoskeleton-dependent cell cycle, migration and invasion of human osteosarcoma cells through NF-?B pathway-related mechanisms. Oncol Rep 44:2621-2633 (2020). PubMed: 33125106
- Venit T et al. Transcriptional Profiling Reveals Ribosome Biogenesis, Microtubule Dynamics and Expression of Specific lncRNAs to be Part of a Common Response to Cell-Penetrating Peptides. Biomolecules 10:N/A (2020). PubMed: 33213097
- Pocasap P et al. Alyssin and Iberin in Cruciferous Vegetables Exert Anticancer Activity in HepG2 by Increasing Intracellular Reactive Oxygen Species and Tubulin Depolymerization. Biomol Ther (Seoul) 27:540-552 (2019). PubMed: 31405267
- Pocasap P et al. Structures of isothiocyanates attributed to reactive oxygen species generation and microtubule depolymerization in HepG2 cells. Biomed Pharmacother 101:698-709 (2018). PubMed: 29522950