重组Alexa Fluor® 488荧光Anti-TROP2抗体[EPR20043]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TROP2 with ab281933 at 1/100 (5.0 μg/ml) dilution.

Membrane staining on human breast.

The section was incubated with ab281933 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TROP2 with ab281933.

Positive staining on human breast. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281933 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TROP2 with ab281933 at 1/50 dilution.

Negative control : no staining on human testis. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281933 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling TROP2 with ab281933 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor^® 488 (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling TROP2 with ab281933 at 1/50 dilution (10.0 μg/ml) (Green).

Confocal image showing membranous staining in MCF7 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^®. 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TROP2 with ab281933 at 1/100 (5 μg/ml) dilution.

Negative control : no staining on human testis.

The section was incubated with ab281933 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TROP2 with ab281933 at 1/50 (10 μg/ml) dilution.

Positive staining on mouse skin.

The section was incubated with ab281933 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded mouse breast cancer tissue labeling TROP2 with ab281933 at 1/50 (10 μg/ml) dilution.

Positive staining on mouse breast cancer.

The section was incubated with ab281933 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TROP2 with ab281933 at 1/50 (10 μg/ml) dilution.

Negative control : no staining on mouse cardiac muscle.

The section was incubated with ab281933 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TROP2 antibody [EPR20043] (AB281933)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TROP2 with ab281933 at 1/50 (10 μg/ml) dilution.

Negative control : no staining on mouse liver.

The section was incubated with ab281933 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.