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重组RabMAb

重组Alexa Fluor® 488荧光Anti-TRAP/CD40L抗体[EPR20727-307] (ab275203)

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Flow Cytometry - Alexa Fluor® 488 Anti-TRAP/CD40L antibody [EPR20727-307] (ab275203)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Alexa Fluor® 488 Rabbit monoclonal [EPR20727-307] to TRAP/CD40L
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 647 Unconjugated

    概述

    • 产品名称

      Alexa Fluor® 488荧光Anti-TRAP/CD40L抗体[EPR20727-307]
      参阅全部 TRAP/CD40L 一抗

    • 描述

      Alexa Fluor® 488荧光兔单克隆抗体[EPR20727-307] to TRAP/CD40L

    • 宿主

      Rabbit

    • 偶联物

      Alexa Fluor® 488. Ex: 495nm, Em: 519nm

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • Flow Cyt: Human PBMCs with PMA (50ng/ml) and Ionomycin (500ng/ml).

    • 常规说明

      This antibody clone is manuactured by Abcam. If you require a custom buffer formulation or conjugation for experiments, please contact orders@abcam.com. 

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab275203于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    1/500.
    说明
    Flow Cyt
    1/500.

    靶标

    • 功能

      Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

    • 组织特异性

      Specifically expressed on activated CD4+ T-lymphocytes.

    • 疾病相关

      Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.

    • 序列相似性

      Belongs to the tumor necrosis factor family.

    • 翻译后修饰

      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.

    • 细胞定位

      Secreted and Cell membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD 40L antibody
        • CD154 antibody
        • CD40 antigen ligand antibody
        • CD40 ligand antibody
        • CD40 ligand, soluble form antibody
        • CD40-L antibody
        • CD40L antibody
        • CD40L_HUMAN antibody
        • CD40LG antibody
        • gp39 antibody
        • hCD40L antibody
        • HIGM1 antibody
        • IGM antibody
        • IMD3 antibody
        • T B cell activating molecule antibody
        • T BAM antibody
        • T-cell antigen Gp39 antibody
        • TNF-related activation protein antibody
        • TNFSF5 antibody
        • TrAP antibody
        • Tumor necrosis factor (ligand) superfamily member 5 antibody
        • Tumor necrosis factor ligand superfamily member 5 antibody
        see all

      图片

      • Flow Cytometry - Alexa Fluor® 488 Anti-TRAP/CD40L antibody [EPR20727-307] (ab275203)
        Flow Cytometry - Alexa Fluor® 488 Anti-TRAP/CD40L antibody [EPR20727-307] (ab275203)

        Flow cytometry staining of  human peripheral blood mononuclear cells (PBMCs) (top), or PBMCs treated with PMA (50 ng/ml, 18h) and ionomycin (500 ng/ml, 18h) (bottom) with ab275203 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 488  (ab199091) isotype control (left). PBMCs were incubated on ice in 1x PBS containing 10 µg/ml anti CD16/CD32 and 10 % normal goat serum followed by the antibody (ab275203) or  Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) isotype (1x 106 in 100 µl at 1 µg/ml (1/500)) for 30min on ice. The cells were also stained for CD4.

        Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

        Events were gated on live lymphocytes.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (0)

      发表研究结果有使用 ab275203？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab275203 尚未被引用在任何文献中。

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