重组Alexa Fluor® 488荧光Anti-Transferrin Receptor抗体[EPR20584] (ab317529)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)](https://www.abcam.cn/ps/products/317/ab317529/Images/ab317529-4-alexa-fluor-488-anti-transferrin-receptor-antibody-epr20584-immunohistochemistry-human-cerebrum.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR20584] to Transferrin Receptor
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-Transferrin Receptor抗体[EPR20584]
参阅全部 Transferrin Receptor 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR20584] to Transferrin Receptor -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human cerebrum, Human placenta, Human stomach cancer, Mouse cerebrum. ICC/IF: K-562 (human chronic myelogenous leukemia lymphoblast), Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20584 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317529于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/50.
|
| 说明 |
|---|
|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/50. |
靶标
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功能
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
疾病相关
Immunodeficiency 46 -
序列相似性
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
翻译后修饰
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
细胞定位
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- Unigene: 529618 Human
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别名
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.
Positive staining on capillaries of human cerebrum.The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)](https://www.abcam.cn/ps/products/317/ab317529/Images/ab317529-5-alexa-fluor-488-anti-transferrin-receptor-antibody-epr20584-immunohistochemistry-human-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.
Positive staining on human placental trophoblast.The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)](https://www.abcam.cn/ps/products/317/ab317529/Images/ab317529-6-alexa-fluor-488-anti-transferrin-receptor-antibody-epr20584-immunohistochemistry-human-stomach-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.
Cytoplasmic staining on human stomach cancer.The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)](https://www.abcam.cn/ps/products/317/ab317529/Images/ab317529-7-alexa-fluor-488-anti-transferrin-receptor-antibody-epr20584-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.
Positive staining on capillaries of mouse cerebrum.The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)](https://www.abcam.cn/ps/products/317/ab317529/Images/ab317529-2-alexa-fluor-488-anti-transferrin-receptor-antibody-epr20584-immunocytochemistry-k-562.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and membranous staining in K-562 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)](https://www.abcam.cn/ps/products/317/ab317529/Images/ab317529-3-alexa-fluor-488-anti-transferrin-receptor-antibody-epr20584-immunocytochemistry-raw-2647.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and membranous staining in Raw 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
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![Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)](https://www.abcam.cn/ps/products/317/ab317529/Images/ab317529-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (ab317529)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317529 尚未被引用在任何文献中。