重组Alexa Fluor® 488荧光Anti-TMEM119抗体[106-6] (ab225497)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)](https://www.abcam.cn/ps/products/225/ab225497/Images/ab225497-331307-anti-tmem119-antibody-106-6-alexa-fluor-488-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [106-6] to TMEM119
- Suitable for: Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-TMEM119抗体[106-6]
参阅全部 TMEM119 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[106-6] to TMEM119 -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8R138 -
阳性对照
- Flow Cyt (Intra): HEK-293T transfected with Myc-His tagged TMEM119 expression vector; CD11b+ brain cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
106-6 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
- Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
- Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (ab220249)
- Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494)
- PE Anti-TMEM119 antibody [106-6] (ab225496)
- Anti-TMEM119 antibody [28-3] - BSA and Azide free (ab234501)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. This antibody may be used at 10 µg/mL. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. This antibody may be used at 10 µg/mL. |
靶标
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细胞定位
Membrane; Single-pass type I membrane protein -
数据库链接
- Entrez Gene: 338773 Human
- Entrez Gene: 231633 Mouse
- SwissProt: Q4V9L6 Human
- SwissProt: Q8R138 Mouse
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别名
- OBIF antibody
- Osteoblast induction factor antibody
- PSEC0199 antibody
see all
图片
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)](https://www.abcam.cn/ps/products/225/ab225497/Images/ab225497-369635-anti-tmem119-antibody-106-6-alexa-fluor488-flow-cytometry-cd11b-mouse.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)This image is courtesy of an anonymous collaborator.Flow cytometric analysis of wild type (WT) versus Tmem119 knockout (KO) CD11b+ cells labeling TMEM119 with ab225497 at 10 μg/mL (green) or the same clone unconjugated (ab210405) at 1 μg/mL with an Alexa 488 secondary antibody (purple), compared with TMEM119 KO primary mouse brain cells stained with ab210405 at 1 μg/mL (black) and ab225497 at 10 μg/mL (grey). Goat anti-Rabbit IgG (Alexa Fluor®488) at 1:1000 dilution was used as the secondary antibody.
No signal was detected on the surface of CD11b+ brain cells from TMEM119 KO mouse stained with either the unconjugated ab210405 (black) or conjugated ab225497 antibodies (grey); whereas in wildtype CD11b+ brain cells, cell surface staining was observed (green, Alexa conjugated ab225497; purple, unconjugated ab210405 plus secondary antibody).
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![Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)](https://www.abcam.cn/ps/products/225/ab225497/Images/ab225497-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab225497 被引用在 1 文献中.
- Marini C et al. The Role of Endoplasmic Reticulum in the Differential Endurance against Redox Stress in Cortical and Spinal Astrocytes from the Newborn SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis. Antioxidants (Basel) 10:N/A (2021). PubMed: 34573024