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AB323448

Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5]

Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5]

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Rabbit Recombinant Monoclonal TCF7 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Human samples.

查看别名

TCF1, TCF7, Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5] (AB323448)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5] (AB323448)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TCF7 with ab323448 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human skeletal muscle.
The section was incubated with ab323448 for 60 mins at room temperature. Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5] (AB323448)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5] (AB323448)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TCF7 with ab323448 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human tonsil.
The section was incubated with ab323448 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5] (AB323448)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TCF7 antibody [EPR28579-5] (AB323448)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling TCF7 with ab323448 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human Hodgkin's Lymphoma.
The section was incubated with ab323448 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

不同偶联物与剂型 (3)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR28579-5

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

反应种属

Human, Human

应用

Flow Cyt (Intra), IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

<p>This antibody should detect all TCF1/TCF7 isoforms, including those lacking the amino-terminal β-catenin binding domain.</p>

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搭配使用产品

Primary Antibodies

AB225472

Alexa Fluor® 488 Anti-Granzyme B antibody [EPR20129-217]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Granzyme B antibody [EPR20129-217] (AB225472)

  • 0
  • 0
Primary Antibodies

AB313665

Alexa Fluor® 488 Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR19518] (AB313665)

  • 0
  • 0
Primary Antibodies

AB281847

Alexa Fluor® 488 Anti-Ki67 antibody [SP6]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)

  • 5
  • 1
Primary Antibodies

AB317716

Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16]

Recombinant|RabMAb

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (AB317716)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TCF7 also known as TCF1 is a transcription factor belonging to the TCF/LEF family with a molecular mass of approximately 54 kDa. TCF7 plays a significant mechanical role in binding to DNA and regulating gene expression in cell nuclei. It interacts with beta-catenin to control the transcription of target genes. This protein is expressed in various tissues including the thymus and other parts of the immune system as well as the intestines.
Biological function summary

TCF7 regulates cell fate decisions and differentiation specifically in the T-cell lineage. It is part of a protein complex with beta-catenin and acts as a transcriptional activator. TCF7 influences the development and function of T-cells playing a critical role in adaptive immune response. It contributes to the maintenance of stem cell properties in various tissues impacting processes like cell proliferation and survival.

Pathways

TCF7 is a vital component of the Wnt/beta-catenin signaling pathway which is essential for regulating gene expression in various cellular processes. This pathway maintains homeostasis in cell proliferation differentiation and stem cell renewal. Within this signaling network beta-catenin an important mediator partners with TCF7 to modulate target gene transcription. TCF7 also interacts with other proteins such as LEF1 which similarly participates in the transcription regulation vital for cellular development.

TCF7 associates with several pathological conditions including cancers and immunodeficiencies. Abnormal activation of the Wnt/beta-catenin pathway involving TCF7 contributes to the development of colon cancer by promoting unregulated cell division. TCF7 mutations have been linked to immunodeficiency disorders given its critical role in T-cell development. These mutations may disrupt normal T-cell formation leading to impaired immune responses.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.
See full target information TCF7
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Abcam Product Promise

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