重组Alexa Fluor® 488荧光Anti-Syntaxin 16抗体[EPR9156] (ab225187)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Syntaxin 16 antibody [EPR9156] (ab225187)](https://www.abcam.cn/ps/products/225/ab225187/Images/ab225187-319941-ICCAP00406361.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Syntaxin 16 antibody [EPR9156] (ab225187)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR9156] to Syntaxin 16
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-Syntaxin 16抗体[EPR9156]
参阅全部 Syntaxin 16 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR9156] to Syntaxin 16 -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9156 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225187于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
| 说明 |
|---|
|
ICC/IF
1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
靶标
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功能
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. -
组织特异性
Ubiquitous. -
疾病相关
Genetic variations in STX16 may be a cause of pseudohypoparathyroidism type 1B (PHP1B) [MIM:603233]. Pseudohypoparathyroidism refers to a heterogeneous group of disorders characterized by resistance to parathyroid hormone (PTH). PHP1B is characterized by PTH-resistant hypocalcemia and hyperphosphatemia. Patients affected with PHP1B lack developmental defects characteristic of Albright hereditary osteodystrophy, and typically show no other endocrine abnormalities besides resistance to PTH. In some cases microdeletions involving STX16 appear to cause loss of methylation at exon A/B of the GNAS gene, resulting in PHP1B. -
序列相似性
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
细胞定位
Cytoplasm and Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8675 Human
- Entrez Gene: 228960 Mouse
- Entrez Gene: 362283 Rat
- Omim: 603666 Human
- SwissProt: O14662 Human
- SwissProt: Q8BVI5 Mouse
- Unigene: 307913 Human
- Unigene: 277977 Mouse
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别名
- hsyn16 antibody
- MGC90328 antibody
- Stx16 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Syntaxin 16 antibody [EPR9156] (ab225187)
ab225187 staining Syntaxin 16 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225187 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 488 Anti-Syntaxin 16 antibody [EPR9156] (ab225187)](https://www.abcam.cn/ps/products/225/ab225187/Images/ab225187-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Syntaxin 16 antibody [EPR9156] (ab225187)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225187 尚未被引用在任何文献中。