AB199767

重组Alexa Fluor® 488荧光Anti-STAT5b抗体[EPR16671]

Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal STAT5b antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

Signal transducer and activator of transcription 5B, STAT5B

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671] (AB199767)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671] (AB199767)

ab199767 staining STAT5b in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671] (AB199767)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671] (AB199767)

Overlay histogram showing HeLa cells stained with ab199767 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab199767, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

不同偶联物与剂型 (1)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-STAT5b antibody [EPR16671]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR16671

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

STAT5b also known as Signal Transducer and Activator of Transcription 5B is a 92-kDa protein involved in signaling processes within the cell. Mechanically it functions as a transcription factor and shuttles between the cytoplasm and nucleus modulating gene expression according to extracellular signals. Upon cytokine binding STAT5b undergoes phosphorylation allowing it to form dimers and translocate to the nucleus. It is expressed in many tissues including the liver immune cells and mammary glands reflecting its role in diverse physiological processes.

Biological function summary

STAT5b plays an important role in regulating cell growth differentiation and apoptosis. It often acts as part of the STAT protein family binding to specific DNA sequences to activate transcription. STAT5b particularly influences immune function and hematopoiesis driving proliferation and differentiation of lymphoid and myeloid cells. Its activity affects glucose homeostasis growth hormone signaling and lactation highlighting its importance in various physiological states and developmental processes.

Pathways

STAT5b integrates into the JAK-STAT signaling pathway a critical route for conveying extracellular information to the nucleus. This pathway includes JAK kinases which activate STAT5b by phosphorylation upon cytokine-receptor engagement. STAT5b also participates in the growth hormone pathway interacting with proteins such as Growth Hormone Receptor and other STAT family members like STAT3. Through these interactions STAT5b helps regulate gene expression associated with growth and differentiation in response to hormonal signals.

STAT5b links to several conditions such as growth hormone insensitivity and leukemia. In growth disorders defects in STAT5b can impair normal growth hormone signal transduction often involving interplay with proteins like the Growth Hormone Receptor. In leukemias aberrant activation or expression of STAT5b contributes to uncontrolled cellular proliferation often accompanied by deregulation of the JAK-STAT pathway. Research into STAT5b offers insights into potential therapeutic targets for these and other related conditions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Carries out a dual function : signal transduction and activation of transcription (PubMed : 29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.

See full target information STAT5B

style

left-column