AB302972

重组Alexa Fluor® 488荧光Anti-Pancreatic Polypeptide抗体[EPR23320-10]

Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Pancreatic Polypeptide antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

查看别名

PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue stained for Pancreatic Polypeptide using ab302972 at a 1/100 dilution (5.0 μg/ml) (Green). Anti-PPY stained on pancreatic islets shown in green; anti-PGP9.5 (ab196173) stained on pancreatic islets shown in red. The section was incubated with ab302972 and ab196173 at 4°C overnight (shown in green and red respectively). Nuclear DNA was labelled with DAPI (Blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for Pancreatic Polypeptide using ab302972 at a 1/100 dilution (5.0 μg/ml) (Green). Positive staining on mouse pancreatic islet. The section was incubated with ab302972 at 4°C overnight (Green). Nuclear DNA was labelled with DAPI (Blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for Pancreatic Polypeptide using ab302972 at a 1/100 dilution (5.0 μg/ml) (Green). Positive staining on rat pancreatic islet. The section was incubated with ab302972 at 4°C overnight (Green). Nuclear DNA was labelled with DAPI (Blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.

不同偶联物与剂型 (3)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10]
Unconjugated

Anti-Pancreatic Polypeptide antibody [EPR23320-10]
Carrier free

Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23320-10

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic Polypeptide also known as PP is a small protein with a molecular mass approximately 4200 Da. It is secreted by PP cells in the pancreas particularly in the F-cells located in the Langerhans islets. This peptide is highly conserved among different species including the rat where it displays similar function and expression patterns. Pancreatic polypeptide regulates various physiological processes by binding to specific receptors on target tissues.

Biological function summary

In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.

Pathways

Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.

Pancreatic polypeptide levels relate to conditions such as obesity and diabetes. Altered secretion of this peptide can contribute to obesity by affecting appetite regulation and energy homeostasis. Furthermore in diabetes pancreatic polypeptide is connected to abnormal pancreatic function often observed alongside insulin and glucagon dysregulation. Understanding these relationships can provide insight into therapeutic strategies targeting metabolic and endocrine disorders.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Pancreatic polypeptide. Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.

See full target information PPY

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Alexa Fluor® 488荧光Anti-Pancreatic Polypeptide抗体[EPR23320-10]