重组Alexa Fluor® 488荧光Anti-Olig2抗体[EPR2673] (ab225099)
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)](https://www.abcam.cn/ps/products/225/ab225099/Images/ab225099-544793-alexa-fluor-488-anti-olig2-antibody-epr2673-immunohistochemistry-frozen-mouse.jpg "Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR2673] to Olig2
- Suitable for: IHC-Fr
- Reacts with: Mouse, Rat
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-Olig2抗体[EPR2673]
参阅全部 Olig2 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR2673] to Olig2 -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-Fr: Normal rat brain tissue sections, mouse cerebrum (fresh frozen).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2673 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Olig2 antibody [EPR2673] (ab109186)
- Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
- Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] (ab225100)
- Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (ab300729)
- Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673] (ab300730)
- Alexa Fluor® 568 Anti-Olig2 antibody [EPR2673] (ab302808)
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225099于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
1/50 - 1/250.
|
| 说明 |
|---|
|
IHC-Fr
1/50 - 1/250. |
靶标
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功能
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. -
组织特异性
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. -
疾病相关
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
结构域
The bHLH is essential for interaction with NKX2-2. -
细胞定位
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. - Information by UniProt
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数据库链接
- Entrez Gene: 10215 Human
- Entrez Gene: 50913 Mouse
- Entrez Gene: 304103 Rat
- Omim: 606386 Human
- SwissProt: Q13516 Human
- SwissProt: Q9EQW6 Mouse
- Unigene: 176977 Human
- Unigene: 37289 Mouse
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别名
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh frozen) tissue labelling Olig2 with ab225099 at 1/50 dilution.
The nuclear counterstain was DAPI (blue).
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![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)](https://www.abcam.cn/ps/products/225/ab225099/Images/ab225099-317921-anti-olig2-antibody-epr2673-alexa-fluor-488-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)IHC image of Olig2 staining in a section of frozen normal rat brain.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225099 at 1/250 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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![Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)](https://www.abcam.cn/ps/products/225/ab225099/Images/ab225099-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab225099 被引用在 1 文献中.
- Szczygiel JA et al. Gene Therapy Vector Encoding Neuropeptide Y and Its Receptor Y2 for Future Treatment of Epilepsy: Preclinical Data in Rats. Front Mol Neurosci 13:232 (2020). PubMed: 33343295