重组Alexa Fluor® 488荧光Anti-Mesothelin抗体[EPR19025-42]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Mesothelin antibody [EPR19025-42] (AB315357)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling Mesothelin with ab315357 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human skeletal muscle.The section was incubated with ab315357 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Mesothelin antibody [EPR19025-42] (AB315357)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Mesothelin with ab315357 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human ovarian carcinoma. The section was incubated with ab315357 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• Flow Cyt

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Flow Cytometry - Alexa Fluor® 488 Anti-Mesothelin antibody [EPR19025-42] (AB315357)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling Mesothelin with ab315357 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : MCF7 (PMID : 18245228). Gated on viable cell.