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重组RabMAb

重组Alexa Fluor® 488荧光Anti-MAdCAM1抗体[EPR27223-58] (ab318243)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)
  • Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR27223-58] to MAdCAM1
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

概述

  • 产品名称

    Alexa Fluor® 488荧光Anti-MAdCAM1抗体[EPR27223-58]
    参阅全部 MAdCAM1 一抗

  • 描述

    Alexa Fluor® 488荧光兔单克隆抗体[EPR27223-58] to MAdCAM1

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Peripheral lymph nodes in Mouse jejunum and peripheral lymph nodes in Rat jejunum.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318243于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

  • 组织特异性

    Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

  • 序列相似性

    Contains 2 Ig-like (immunoglobulin-like) domains.

  • 翻译后修饰

    The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Addressin mucosal antibody
      • hMAdCAM 1 antibody
      • hMAdCAM-1 antibody
      • MACAM1 antibody
      • MADCA_HUMAN antibody
      • MAdCAM 1 antibody
      • MAdCAM-1 antibody
      • Madcam1 antibody
      • Mucosal addressin cell adhesion molecule 1 antibody
      • Mucosal vascular addressin cell adhesion molecule 1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)

      Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MAdCAM1 with ab318243 at 1/100 (5.0 ug/ml) dilution.

      Negative control: No staining on mouse cardiac muscle.
      The section was incubated with ab318243 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Counterstained with DAPI.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)

      Immunohistochemical analysis of paraffin-embedded Rat peripheral lymph tissue labeling MAdCAM1 with ab318243 at 1/100 (5.0 ug/ml) dilution.

      Positive staining on the high endothelial venules of peripheral lymph nodes in rat jejunum.
      The section was incubated with ab318243 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Counterstained with DAPI.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)

      Immunohistochemical analysis of paraffin-embedded Mouse peripheral lymphoid tissue labeling MAdCAM1 with ab318243 at 1/100 (5.0 ug/ml) dilution.

      Positive staining on the high endothelial venules of peripheral lymph nodes in mouse jejunum.
      The section was incubated with ab318243 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Counterstained with DAPI.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

    • Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)
      Alexa Fluor® 488 Anti-MAdCAM1 antibody [EPR27223-58] (ab318243)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab318243？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab318243 尚未被引用在任何文献中。

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