AB225382

重组Alexa Fluor® 488荧光Anti-LC3B抗体[EPR18709] - Autophagosome Marker

Name: Alexa Fluor® 488 Anti-LC3B抗体[EPR18709] - Autophagosome Marker (ab225382)| Abcam中文官网
Brand: Abcam Limited
SKU: AB225382
Price: 5157 CNY
Availability: InStock
Rating: 5 (1 reviews)

Alexa Fluor® 488 Anti-LC3B antibody [EPR18709] - Autophagosome Marker

RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(10 Publications)

Rabbit Recombinant Monoclonal LC3B antibody - conjugated to Alexa Fluor® 488. Autophagosome marker. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

查看别名

MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, Microtubule-associated proteins 1A/1B light chain 3B, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB225382)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB225382)

ab225382 staining LC3B in HeLa chloroquine-treated (50μM, 24 hours) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225453 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB225382)

ab225383 staining LC3B in wild-type HAP1 cells and knockout cells, untreated and chloroquine-treated (50μM, 24 hours). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab225382 at 0.5μg/ml (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

不同偶联物与剂型 (4)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709]
Unconjugated

Anti-LC3B antibody [EPR18709] - Autophagosome Marker
Carrier free

Anti-LC3B antibody [EPR18709] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR18709

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human, Human, Mouse, Rat

应用

ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "This product gave a positive signal in chloroquine- treated HeLa cells and chloroquine-treated HAP1 cells fixed with 100% methanol (5 min)", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).

See full target information MAP1LC3B

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadv8640 PubMed40512859

2025

Interaction between NF-κB and PLAC8 impairs autophagy providing a survival advantage to prostate cells transformed by cadmium.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Shukla,Ashish Tyagi,Balaji Chandrasekaran,Bhawna Tyagi,Balpreet Singh,Thulasidharan Nair Devanarayanan,Venkatesh Kolluru,Murali K Ankem,Chendil Damodaran

Materials today. Bio 31:101610 PubMed40104642

2025

Injectable sustainable andrographolide-releasing hydrogel for long-lasting alleviation of osteoarthritis and regulation of chondrocyte autophagy via PRKCA/EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Chen,Peipei He,Siyi Tao,Jintao Zhong,Kai Jiang,Yuching Hsu,Guang Xia,Xinzhan Mao,Hongxun Sang,Ke Lu

Nature communications 16:1358 PubMed39905002

2025

Targeting ceramide transfer protein sensitizes AML to FLT3 inhibitors via a GRP78-ATF6-CHOP axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofan Sun,Yue Li,Juan Du,Fangshu Liu,Caiping Wu,Weihao Xiao,Guopan Yu,Xiaowei Chen,Robert Peter Gale,Hui Zeng

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

Experimental and therapeutic medicine 27:252 PubMed38682112

2024

GDF11 inhibits the malignant progression of hepatocellular carcinoma via regulation of the mTORC1‑autophagy axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyi Wu,Chan Fan,Kebo Liu,Jiefu Tang

Journal of inflammation research 17:1397-1411 PubMed38476473

2024

Identification and Validation of Genes Related to Macrophage Polarization and Cell Death Modes Under Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zisha Yang,Jiajun Wang,Jiang Pi,Di Hu,Junfa Xu,Yi Zhao,Yan Wang

The FEBS journal 290:4562-4576 PubMed37255287

2023

PTPN22 activates the PI3K pathway via 14-3-3τ in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Bai,Tao Wang,Xiaonan Zhang,Xinlei Ba,Na Zhang,Yanjiao Zhao,Xipeng Wang,Yang Yu,Bing Wang

Scientific reports 13:6393 PubMed37076504

2023

Dentin defects caused by a Dspp frameshift mutation are associated with the activation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Charles E Smith,Yuanyuan Hu,Hong Zhang,Chuhua Zhang,Qian Xu,Yongbo Lu,Ling Qi,Jan C-C Hu,James P Simmer

Cancer discovery 13:1186-1209 PubMed36811466

2023

Addressing Tumor Heterogeneity by Sensitizing Resistant Cancer Cells to T cell-Secreted Cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinaga Ito,Deng Pan,Wubing Zhang,Xixi Zhang,Tiffany Y Juan,Jason W Pyrdol,Oleksandr Kyrysyuk,John G Doench,X Shirley Liu,Kai W Wucherpfennig

Dose-response : a publication of International Hormesis Society 19:15593258211011023 PubMed34035782

2021

Tenacissoside H Induces Autophagy and Radiosensitivity of Hepatocellular Carcinoma Cells by PI3K/Akt/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiatian Lin,Jiyin Ruan,Hao Zhu,Zaizhong Chen,Junhui Chen,Hongjian Yu

View all publications

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重组Alexa Fluor® 488荧光Anti-LC3B抗体[EPR18709] - Autophagosome Marker