重组Alexa Fluor® 488荧光Anti-KMT6 / EZH2抗体[EPR9307(2)] (ab225362)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [EPR9307(2)] (ab225362)](https://www.abcam.cn/ps/products/225/ab225362/Images/ab225362-318229-anti-kmt6-ezh2-antibody-epr93072-alexa-fluor-488-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [EPR9307(2)] (ab225362)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR9307(2)] to KMT6 / EZH2
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-KMT6 / EZH2抗体[EPR9307(2)]
参阅全部 KMT6 / EZH2 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR9307(2)] to KMT6 / EZH2 -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: Jurkat cells. Flow Cyt (intra): Jurkat cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9307(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225362于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) |
|
| Flow Cyt (Intra) |
1/50.
|
| 说明 |
|---|
|
ICC/IF
1/100. This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) |
|
Flow Cyt (Intra)
1/50. |
靶标
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功能
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. -
组织特异性
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. -
序列相似性
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
发展阶段
Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. -
翻译后修饰
Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2146 Human
- Entrez Gene: 14056 Mouse
- Entrez Gene: 312299 Rat
- Omim: 601573 Human
- SwissProt: Q15910 Human
- SwissProt: Q61188 Mouse
- Unigene: 444082 Human
- Unigene: 246688 Mouse
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别名
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
see all
图片
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [EPR9307(2)] (ab225362)
Overlay histogram showing Jurkat cells stained with ab225362 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225362, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [EPR9307(2)] (ab225362)](https://www.abcam.cn/ps/products/225/ab225362/Images/ab225362-295560-anti-kmt6-ezh2-antibody-epr93072-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [EPR9307(2)] (ab225362)ab225362 staining KMT6 / EZH2 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225362 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [EPR9307(2)] (ab225362)](https://www.abcam.cn/ps/products/225/ab225362/Images/ab225362-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-KMT6 / EZH2 antibody [EPR9307(2)] (ab225362)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225362 尚未被引用在任何文献中。