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重组RabMAb

重组Alexa Fluor® 488荧光Anti-IL-17A抗体[EPR26410-51] (ab317361)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)
  • Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR26410-51] to IL-17A
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

概述

  • 产品名称

    Alexa Fluor® 488荧光Anti-IL-17A抗体[EPR26410-51]
    参阅全部 IL-17A 一抗

  • 描述

    Alexa Fluor® 488荧光兔单克隆抗体[EPR26410-51] to IL-17A

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: EL4 cells treated with Phorbol-12-myristate-13-acetate (100 ng/mL) and Brefeldin A (500 ng/mL) for overnight. Flow Cyt (intra): EL4 treated with 50ng/ml PMA and 500ng/ml Ionomycin Ca2+ Salt and 500ng/ml Brefeldin A overnight.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • 存储溶液

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR26410-51

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317361于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/50.
Flow Cyt (Intra)
1/500.
说明
ICC/IF
1/50.
Flow Cyt (Intra)
1/500.

靶标

  • 功能

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.

  • 组织特异性

    Restricted to activated memory T-cells.

  • 序列相似性

    Belongs to the IL-17 family.

  • 翻译后修饰

    Found both in glycosylated and nonglycosylated forms.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CTLA 8 antibody
      • CTLA-8 antibody
      • CTLA8 antibody
      • cytotoxic T lymphocyte associated antigen 8 antibody
      • Cytotoxic T lymphocyte associated protein 8 antibody
      • Cytotoxic T lymphocyte associated serine esterase 8 antibody
      • Cytotoxic T-lymphocyte-associated antigen 8 antibody
      • IL 17 antibody
      • IL 17A antibody
      • IL-17 antibody
      • IL-17A antibody
      • IL17 antibody
      • IL17_HUMAN antibody
      • Il17a antibody
      • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
      • interleukin 17A antibody
      • Interleukin-17A antibody
      • interleukin17 antibody
      • Interleukin17A antibody
      • OTTHUMP00000016597 antibody
      • OTTMUSP00000046003 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling IL-17A with AB317361 at 1/50 (10.0 ug/ml) dilution (Green).

      Confocal image showing increased cytoplasmic staining in EL4 cells treated with Phorbol-12-myristate-13-acetate (100 ng/mL) and Brefeldin A (500 ng/mL) for overnight (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4 (mouse lymphoma T lymphocyte) treated with 50ng/ml PMA and 500ng/ml Ionomycin Ca2+ Salt and 500ng/ml Brefeldin A overnight (Right) / Untreated control (Left) cells labelling IL-17A with AB317361 at 1/500 dilution (0.1ug)/ Right and Left.

    • Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)
      Alexa Fluor® 488 Anti-IL-17A antibody [EPR26410-51] (ab317361)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317361？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317361 尚未被引用在任何文献中。

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