JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB225357

重组Alexa Fluor® 488荧光Anti-hnRNP G抗体[EPR16038]

Alexa Fluor® 488 Anti-hnRNP G antibody [EPR16038]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal hnRNP G antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

HNRPG, RBMXP1, RBMX, Glycoprotein p43, Heterogeneous nuclear ribonucleoprotein G, hnRNP G

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-hnRNP G antibody [EPR16038] (AB225357)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-hnRNP G antibody [EPR16038] (AB225357)

ab225357 staining hnRNP G in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225357 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

不同偶联物与剂型 (3)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR16038

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

反应种属

Human

应用

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein G (hnRNP G) also referred to as RBMX is a protein involved in RNA binding and processing. It is a member of the hnRNP protein family recognized for its role in managing the intricate processes of RNA metabolism. hnRNP G has a mass of approximately 43 kDa and is widely expressed in various tissues. The protein typically resides in the nucleus but it can shuttle between the nucleus and cytoplasm a common feature in the hnRNP family aiding in its diverse functionality.
Biological function summary

HnRNP G influences mRNA splicing stability and transport. It does not operate in isolation; instead it is part of ribonucleoprotein complexes that include other hnRNPs that work collectively. This protein facilitates alternative splicing events contributing to the generation of different protein isoforms that are essential for cellular diversity and adaptation. The ability of hnRNP G to bind to RNA and participate in these cellular events highlights its significance in regulatory processes.

Pathways

HnRNP G plays a role in the regulation of gene expression. It is an important player in the alternative splicing pathway affecting the diversity of mRNA products. hnRNP G interacts with the splicing machinery and can influence splice site selection. This protein is also associated with the DNA damage response pathway where it interacts with other proteins such as BRCA1 particularly under stress conditions that require enhanced DNA repair mechanisms.

HnRNP G has connections to certain types of cancer and neurological disorders. Altered expression of hnRNP G is observed in breast cancer suggesting its role in tumor progression and gene regulation disruptions. Additionally changes in hnRNP G activity have been associated with neurodegenerative diseases like spinal muscular atrophy. In these contexts hnRNP G interfaces with proteins such as SMN1 linking its expression and function to disease outcomes and offering potential avenues for therapeutic intervention.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Also plays a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.
See full target information RBMX
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37370835

2023

Enhancement of the Anticancer Ability of Natural Killer Cells through Allogeneic Mitochondrial Transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-Hoon Kim,Mi-Jin Kim,Mina Lim,Jihye Kim,Hyunmin Kim,Chang-Koo Yun,Yun-Joo Yoo,Youngjun Lee,Kyunghoon Min,Yong-Soo Choi
View all publications
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB195377

Anti-ALKBH5 antibody [EPR18958]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH5 antibody [EPR18958] (AB195377)

  • 4
  • 4
Primary Antibodies

AB220163

Anti-YTHDF2 antibody [EPR20318]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-YTHDF2 antibody [EPR20318] (AB220163)

  • 5
  • 3
Primary Antibodies

AB217109

Alexa Fluor® 647 Anti-METTL3 antibody [EPR18810]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-METTL3 antibody [EPR18810] (AB217109)

  • 0
  • 0
Primary Antibodies

AB309096

Anti-METTL14 antibody [EPR27234-62]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-METTL14 antibody [EPR27234-62] (AB309096)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com