重组Alexa Fluor® 488荧光Anti-HMGA2抗体[EPR18114] (ab317357)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-HMGA2 antibody [EPR18114] (ab317357)](https://www.abcam.cn/ps/products/317/ab317357/Images/ab317357-1-alexa-fluor-488-anti-hmga2-antibody-epr18114-immunohistochemistry-human-lung-cancer.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-HMGA2 antibody [EPR18114] (ab317357)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR18114] to HMGA2
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-HMGA2抗体[EPR18114]
参阅全部 HMGA2 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR18114] to HMGA2 -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human lung cancer tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18114 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Buffer
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317357于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. -
疾病相关
Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma. -
序列相似性
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
发展阶段
Expressed predominantly during embryogenesis. -
翻译后修饰
Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8091 Human
- Omim: 600698 Human
- SwissProt: P52926 Human
- Unigene: 505924 Human
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别名
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-HMGA2 antibody [EPR18114] (ab317357)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling HMGA2 with AB317357 at 1/50 (10.0 ug/ml) dilution.
Nuclear staining on human lung cancer. The section was incubated with ab317357 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-HMGA2 antibody [EPR18114] (ab317357)](https://www.abcam.cn/ps/products/317/ab317357/Images/ab317357-2-alexa-fluor-488-anti-hmga2-antibody-epr18114-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-HMGA2 antibody [EPR18114] (ab317357)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HMGA2 with AB317357 at 1/50 (10.0 ug/ml) dilution.
Negative control: no staining on human colon. The section was incubated with ab317357 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Alexa Fluor® 488 Anti-HMGA2 antibody [EPR18114] (ab317357)](https://www.abcam.cn/ps/products/317/ab317357/Images/ab317357-3-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 488 Anti-HMGA2 antibody [EPR18114] (ab317357)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317357 尚未被引用在任何文献中。