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AB237340

重组Alexa Fluor® 488荧光Anti-Histone H3 (tri methyl K4)抗体[EPR20551-225]

Alexa Fluor® 488 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225]

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(1 Publication)

Rabbit Recombinant Monoclonal H3 tri methyl K4 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3, H3K4me, H3K4

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] (AB237340)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] (AB237340)

Overlay histogram showing NIH/3T3 cells stained with ab237340 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237340) (1x 106 in 100 μl at 0.2 μg/ml (1/2500 dilution)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

This antibody gave a positive signal in NIH/3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] (AB237340)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] (AB237340)

ab237340 staining Histone H3 (tri methyl K4) - ChIP Grade in NIH/3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237340 at 1/1000 dilution (shown in green) and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in NIH/3T3 cells fixed with 4% formaldehyde (10 min).

不同偶联物与剂型 (6)

  • Unconjugated

    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade

  • Mouse version

    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric)

  • Mouse version

    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free

  • Carrier free

    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225]

  • 578 PE

    PE Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20551-225

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

反应种属

Human, Mouse, Rat

应用

Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

AB245911

Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

  • 5
  • 1
Primary Antibodies

AB207543

Alexa Fluor® 647 Anti-Histone H3 antibody [EPR16987] - Nuclear Marker

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Histone H3 antibody [EPR16987] - Nuclear Marker (AB207543)

  • 0
  • 0
Primary Antibodies

AB283349

Alexa Fluor® 647 Anti-Histone H3 (mono methyl K4) antibody [ERP16597]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (mono methyl K4) antibody [ERP16597] (AB283349)

  • 0
  • 0
Primary Antibodies

AB270163

Alexa Fluor® 647 Anti-Histone H3 (tri methyl K27) antibody [EPR18607]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (tri methyl K27) antibody [EPR18607] (AB270163)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in NIH3T3 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 tri methyl K4
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102304 PubMed39281707

2024

Nanobody against SARS-CoV-2 non-structural protein Nsp9 inhibits viral replication in human airway epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Venit,Jeremy Blavier,Sibusiso B Maseko,Sam Shu,Lilia Espada,Christopher Breunig,Hans-Peter Holthoff,Sabrina C Desbordes,Martin Lohse,Gennaro Esposito,Jean-Claude Twizere,Piergiorgio Percipalle
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com