AB245911

重组Alexa Fluor® 488荧光Anti-Histone H3 (acetyl K27)抗体[EP16602]

Name: Alexa Fluor® 488 Anti-Histone H3 (acetyl K27)抗体[EP16602] (ab245911)| Abcam中文官网
Brand: Abcam Limited
SKU: AB245911
Price: 5290 CNY
Availability: InStock
Rating: 5 (1 reviews)

Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602]

BOND RX™ Validated
RabMAb
Recombinant
了解详情

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal H3 acetyl K27 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, Flow Cyt, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27ac

7 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

ab245911 staining Histone H3 (acetyl K27) in HeLa +/- Trichostatin A (500 ng/ml for 4h). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab245911 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^®594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Flow Cytometry - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Flow Cytometry analysis of HeLa (human cervical adenocarcinoma epithelial cell) treated with 500ng/mL TSA for 4 hours (Red) / Untreated HeLa (Dotted Red) cells labeling Histone H3 (acetyl K27) with ab245911 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. ab199091 was used as isotype control (black). Unlabelled control/colour : Cells without incubation with primary antibody and secondary antibody (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry analysis of paraffin-embedded sections human liver cancer labeling Histone H3 (acetyl K27) with ab245911 at 1/100 dilution, incubated with ab245911 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Nuclear staining on human liver cancer.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry analysis of paraffin-embedded sections human colon labeling Histone H3 (acetyl K27) with ab245911 at 1/100 dilution, incubated with ab245911 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Nuclear staining on human colon.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry analysis of paraffin-embedded sections mouse lung labeling Histone H3 (acetyl K27) with ab245911 at 1/100 dilution, incubated with ab245911 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Nuclear staining on mouse lung.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Flow Cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) treated with 500ng/mL TSA for 4 hours (Red) / Untreated NIH/3T3 (Dotted Red) cells labeling Histone H3 (acetyl K27) with ab245911 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. ab199091 was used as isotype control (black). Unlabelled control/colour : Cells without incubation with primary antibody and secondary antibody (blue).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

ab245911 at 1/500 staining Histone H3 (acetyl K27) in NIH/3T3 (mouse embryonic fibroblast) followed by ab150081 at 1/1000 dilution. The cells were fixed with 4% formaldehyde and permeabilized with 0.1% Triton X-100. ab195889 at 1/200 was used as the counterstain antibody. Nuclear DNA was labelled with DAPI (shown in blue). Confocal image showing nuclear staining in NIH/3T3 cells treated with Trichostatin A (500 ng/ml for 4h) (shown in green). The counterstain was observed with red. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

不同偶联物与剂型 (10)

Unconjugated

Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
Mouse version

Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade – Mouse IgG1 (Chimeric)
Mouse version

Anti-Histone H3 (acetyl K27) antibody [EP16602] – Ms IgG1 (Chimeric) – BSA and Azide Free
Carrier free

Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade - BSA and Azide free
660 APC

APC Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H3 (acetyl K27) antibody [EP16602]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
578 PE

PE Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP16602

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

Flow Cyt (Intra), Flow Cyt, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Binds K27ac alone and also when S28 is phosphorylated.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "This product gave a positive signal in Trichostatin A (500ng/ml 4h) treated HeLa fixed with 4% formaldehyde (10 min).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K27

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110668 PubMed39246442

2024

Multiplex imaging of localized prostate tumors reveals altered spatial organization of AR-positive cells in the microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Çiğdem Ak,Zeynep Sayar,Guillaume Thibault,Erik A Burlingame,M J Kuykendall,Jennifer Eng,Alex Chitsazan,Koei Chin,Andrew C Adey,Christopher Boniface,Paul T Spellman,George V Thomas,Ryan P Kopp,Emek Demir,Young Hwan Chang,Vasilis Stavrinides,Sebnem Ece Eksi

Cell reports 43:114054 PubMed38578832

2024

14-3-3 binding motif phosphorylation disrupts Hdac4-organized condensates to stimulate cardiac reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Ienglam Lei,Shuo Tian,Wenbin Gao,Yijing Guo,Zhaokai Li,Ziad Sabry,Paul Tang,Y Eugene Chen,Zhong Wang

Frontiers in medicine 9:897800 PubMed35911406

2022

Establishment of the reproducible branch retinal artery occlusion mouse model and intravital longitudinal imaging of the retinal CX3CR1-GFP cells after spontaneous arterial recanalization.

Applications

Unspecified application

Species

Unspecified reactive species

Jehwi Jeon,Sang-Hoon Kim,Eunji Kong,Soo Jin Kim,Jee Myung Yang,Joo Yong Lee,Junyeop Lee,You-Me Kim,Pilhan Kim

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Alexa Fluor® 488荧光Anti-Histone H3 (acetyl K27)抗体[EP16602]