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AB277918

重组Alexa Fluor® 488荧光Anti-Histone H3 (acetyl K14)抗体[EP964Y]

Alexa Fluor® 488 Anti-Histone H3 (acetyl K14) antibody [EP964Y]

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(1 Publication)

Rabbit Recombinant Monoclonal H3 acetyl K14 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC and reacts with Human samples. Cited in 1 publication.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K14ac

1 Images
Immunocytochemistry - Alexa Fluor® 488 Anti-Histone H3 (acetyl K14) antibody [EP964Y] (AB277918)
  • ICC

Supplier Data

Immunocytochemistry - Alexa Fluor® 488 Anti-Histone H3 (acetyl K14) antibody [EP964Y] (AB277918)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling Histone H3 (acetyl K14) with ab277918 at 1/250 (2.0 μg/ml) dilution.

Confocal image showing increased nuclear staining in HeLa cells transfected with TSA (Trichostatin A) (500ng/ml, 4h).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

不同偶联物与剂型 (7)

  • Biotin

    Biotin Anti-Histone H3 (acetyl K14) antibody [EP964Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Histone H3 (acetyl K14) antibody [EP964Y]

  • 660 APC

    APC Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade

  • 578 PE

    PE Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP964Y

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

反应种属

Human

应用

ICC

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/250", "ICC-species-notes": "<p></p>" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 acetyl K14

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1482-1495 PubMed40921734

2025

Hbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dounia Djeghloul,Sherry Cheriyamkunnel,Bhavik Patel,Holger Kramer,Alex Montoya,Karen E Brown,Chad Whilding,Tatyana B Nesterova,Guifeng Wei,Neil Brockdorff,Iga Grządzielewska,Remzi Karayol,Asifa Akhtar,Matthias Merkenschlager,Amanda G Fisher
View all publications

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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