AB307340

重组Alexa Fluor® 488荧光Anti-Glucagon抗体[EP3070]

Alexa Fluor® 488 Anti-Glucagon antibody [EP3070]

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GLP-1 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

查看别名

Pro-glucagon, GCG

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Glucagon antibody [EP3070] (AB307340)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Glucagon antibody [EP3070] (AB307340)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Glucagon with ab307340 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on human pancreatic islet.The section was incubated with ab307340 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Glucagon antibody [EP3070] (AB307340)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Glucagon with ab307340 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on mouse pancreatic islet.The section was incubated with ab307340 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Glucagon antibody [EP3070] (AB307340)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Glucagon with ab307340 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on rat pancreatic islet.The section was incubated with ab307340 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

不同偶联物与剂型 (7)

Unconjugated

Anti-Glucagon antibody [EP3070]
Rabbit version

Anti-Glucagon [EP3070] – Mouse IgG1 (Chimeric)
Carrier free

Anti-Glucagon antibody [EP3070] - BSA and Azide free
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Glucagon antibody [EP3070]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Glucagon antibody [EP3070]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Glucagon antibody [EP3070]
Biotin

Biotin Anti-Glucagon antibody [EP3070]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP3070

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human, Mouse, Rat, Human, Mouse, Rat

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glucagon also known as the “hunger hormone" is a 29-amino acid peptide hormone with a mass of approximately 3.5 kDa. It plays an important role in glucose metabolism and regulation of blood sugar levels. The human pancreas specifically the alpha cells located in the islets of Langerhans synthesizes and secretes glucagon. This hormone binds to glucagon receptors which are widely expressed across tissues including the liver and kidney where it initiates a cascade of signaling events.

Biological function summary

Glucagon plays a central role in maintaining glucose homeostasis. It increases blood glucose by promoting gluconeogenesis and glycogenolysis in the liver. Though glucagon mainly acts independently it exhibits significant interactions with other metabolic hormones such as insulin. This interaction helps balance blood sugar levels as glucagon and insulin work in opposition to ensure optimal blood glucose regulation.

Pathways

Glucagon primarily impacts the cAMP signaling pathway significantly increasing intracellular cAMP levels. This pathway initiates a complex series of downstream events including increased enzyme activity for gluconeogenesis and glycogen breakdown in the liver. Glucagon also cross-talks with insulin signaling pathways enabling the intertwined regulation of metabolism through these hormones and maintaining glucose balance.

Glucagon is closely linked to diabetes and hyperglycemia. Individuals with diabetes often exhibit dysregulated glucagon secretion leading to unstable blood sugar levels. Glucagon interacts with insulin in the pathology of diabetes where an improper balance between these hormones can exacerbate the disease. The understanding of glucagon's role in these conditions makes it a target for new therapeutic strategies including recombinant glucagon treatments and assays.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Binds to and activates the glucagon receptor GCGR, which couples to the G(s) G protein and elevates intracellular cAMP, triggering downstream metabolic responses (PubMed : 32193322).. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release (PubMed : 22037645, PubMed : 40446798). Also stimulates insulin release in response to IL6 (PubMed : 22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). Has growth-promoting activities on intestinal epithelium (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). Inhibits beta cell apoptosis (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744).. Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

See full target information Pro-glucagon

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重组Alexa Fluor® 488荧光Anti-Glucagon抗体[EP3070]