AB198520

重组Alexa Fluor® 488荧光Anti-Ezrin抗体[EP886Y]

Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Ezrin antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

ab198520 staining Ezrin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198520 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLacells fixed with 4% formaldehyde (10 min).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

Overlay histogram showing SH-SY5Y cells stained with ab198520 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198520, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

不同偶联物与剂型 (1)

HRP

HRP Anti-Ezrin antibody [EP886Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP886Y

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/alexa-fluor-488-rabbit-igg-monoclonal-epr25a-isotype-control-ab199091'>ab199091</a> - Rabbit monoclonal IgG (Alexa FluorÂ® 488), is suitable for use as an isotype control with this antibody.</p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.

Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

See full target information EZR

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 7:12742 PubMed27599456

2016

High cell-surface density of HER2 deforms cell membranes.

Applications

Species

Human

Inhee Chung,Mike Reichelt,Lily Shao,Robert W Akita,Hartmut Koeppen,Linda Rangell,Gabriele Schaefer,Ira Mellman,Mark X Sliwkowski

View all publications

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Abcam Product Promise

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重组Alexa Fluor® 488荧光Anti-Ezrin抗体[EP886Y]