AB199320

重组Alexa Fluor® 488荧光Anti-DcR1抗体[EPR6162]

Alexa Fluor® 488 Anti-DcR1 antibody [EPR6162]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal DcR1 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

CD263, DCR1, LIT, TRAILR3, TRID, UNQ321/PRO366, TNFRSF10C, Tumor necrosis factor receptor superfamily member 10C, Antagonist decoy receptor for TRAIL/Apo-2L, Decoy TRAIL receptor without death domain, Decoy receptor 1, Lymphocyte inhibitor of TRAIL, TNF-related apoptosis-inducing ligand receptor 3, TRAIL receptor without an intracellular domain, DcR1, TRAIL receptor 3, TRAIL-R3

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-DcR1 antibody [EPR6162] (AB199320)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-DcR1 antibody [EPR6162] (AB199320)

ab199320 staining DcR1 in Jurkat cells. The cells were fixed with 80% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199320 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry - Alexa Fluor® 488 Anti-DcR1 antibody [EPR6162] (AB199320)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-DcR1 antibody [EPR6162] (AB199320)

Overlay histogram showing Jurkat cells stained with ab199320 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min.

The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab199320, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

不同偶联物与剂型 (3)

Unconjugated

Anti-DcR1 antibody [EPR6162]
Carrier free

Anti-DcR1 antibody [EPR6162] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-DcR1 antibody [EPR6162]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6162

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human, Human

应用

ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Jurkat cells fixed with 80% methanol (5 min).</p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

This antibody was developed as part of a collaboration with the lab of Shi-Yong Sun at Emory University.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.

See full target information TNFRSF10C

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Plant biotechnology journal 23:615-631 PubMed39585184

2024

The SUMO-conjugating enzyme OsSCE1a from wild rice regulates the functional stay-green trait in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuzhao Yuan,Yanfang Luan,Dong Liu,Jian Wang,Jianxiang Peng,Jinlei Zhao,Lupeng Li,Jingjing Su,Yang Xiao,Yuanjie Li,Xin Ma,Xiaoyang Zhu,Lubin Tan,Fengxia Liu,Hongying Sun,Ping Gu,Ran Xu,Peijiang Zhang,Zuofeng Zhu,Chuanqing Sun,Yongcai Fu,Kun Zhang

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Alexa Fluor® 488荧光Anti-DcR1抗体[EPR6162]