重组Alexa Fluor® 488荧光Anti-Cubilin抗体[EPR16259-27] - C-terminal (ab318235)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab318235)](https://www.abcam.cn/ps/products/318/ab318235/Images/ab318235-2-alexa-fluor-488-anti-cubilin---c-terminal-antibody-epr16259-27-immunohistochemistry-human-skeletal-muscl.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab318235)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR16259-27] to Cubilin - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 488荧光Anti-Cubilin抗体[EPR16259-27] - C-terminal
参阅全部 Cubilin 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR16259-27] to Cubilin - C-terminal -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC-P: Human kidney tissues.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16259-27 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318235于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
-
功能
Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. -
组织特异性
Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney. -
疾病相关
Recessive hereditary megaloblastic anemia 1 -
序列相似性
Contains 27 CUB domains.
Contains 7 EGF-like domains. -
结构域
The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2. -
翻译后修饰
The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off.
N-glycosylated. -
细胞定位
Endosome membrane. Lysosome membrane. Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 8029 Human
- Omim: 602997 Human
- SwissProt: O60494 Human
- Unigene: 166206 Human
-
别名
- 460 kDa receptor antibody
- cubilin (intrinsic factor-cobalamin receptor) antibody
- Cubilin antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab318235)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Cubilin - C-terminal with ab318235 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining on human skeletal muscle.
The section was incubated with ab318235 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab318235)](https://www.abcam.cn/ps/products/318/ab318235/Images/ab318235-1-alexa-fluor-488-anti-cubilin---c-terminal-antibody-epr16259-27-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab318235)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Cubilin - C-terminal with ab318235 at 1/100 (5.0 ug/ml) dilution.
Positive staining on human renal tubules.
The section was incubated with ab318235 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Alexa Fluor® 488 Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab318235)](https://www.abcam.cn/ps/products/318/ab318235/Images/ab318235-3-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 488 Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab318235)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab318235 尚未被引用在任何文献中。