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使用敲除细胞株进行验证重组RabMAb

重组Alexa Fluor® 488荧光Anti-CD73抗体[EPR28213-52] (ab317364)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)
  • Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR28213-52] to CD73
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 568 Alexa Fluor® 647 Carrier Free Unconjugated

概述

  • 产品名称

    Alexa Fluor® 488荧光Anti-CD73抗体[EPR28213-52]
    参阅全部 CD73 一抗

  • 描述

    Alexa Fluor® 488荧光兔单克隆抗体[EPR28213-52] to CD73

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • 经测试应用

    适用于: ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: Wild-type A431 and U-87 MG cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317364于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/50.
说明
ICC/IF
1/50.

靶标

  • 功能

    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

  • 疾病相关

    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.

  • 序列相似性

    Belongs to the 5'-nucleotidase family.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NT5E KO A431 (NT5E knockout human epidermoid carcinoma epithelial cell) cells labelling CD73 with AB317364 at 1/50 (10.0 ug/ml) dilution (Green).

      Confocal image showing membranous staining in A431 cells and no staining in NT5E knockout A431 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labelling CD73 with AB317364 at 1/50 (10.46 ug/ml) dilution (Green).

      Confocal image showing membranous staining in U-87 MG cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Negative control: HeLa (PMID: 8566797) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    • Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)
      Alexa Fluor® 488 Anti-CD73 antibody [EPR28213-52] (ab317364)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317364？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317364 尚未被引用在任何文献中。

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