AB252149

重组Alexa Fluor® 488荧光Anti-CD4抗体[EPR20122]

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal CD4 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples.

查看别名

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

1 Images

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab252149 (right) or Rabbit IgG (monoclonal) Alexa Fluor 488^® (ab199091) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10% mouse serum and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab252149) or Rabbit IgG (monoclonal) Alexa Fluor 488^® (ab199091) isotype (1x 10⁶ in 100μl at 5μg/ml (1/100)) for 30 min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Events were gated on live lymphocytes.

不同偶联物与剂型 (5)

Unconjugated

Anti-CD4 antibody [EPR20122]
Carrier free

Anti-CD4 antibody [EPR20122] - BSA and Azide free
660 APC

APC Anti-CD4 antibody [EPR20122]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD4 antibody [EPR20122]
578 PE

PE Anti-CD4 antibody [EPR20122]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20122

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Mouse, Mouse

应用

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

See full target information Cd4

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Alexa Fluor® 488荧光Anti-CD4抗体[EPR20122]