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AB270139

重组Alexa Fluor® 488荧光Anti-CD16抗体[EPR22409-124]

Alexa Fluor® 488 Anti-CD16 antibody [EPR22409-124]

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Rabbit Recombinant Monoclonal CD16b antibody - conjugated to Alexa Fluor® 488. Suitable for ICC, Flow Cyt and reacts with Human samples.

查看别名

CD16b, CD16B, FCG3, FCGR3, IGFR3, FCGR3B, Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, FcR-10, IgG Fc receptor III-1, CD16-I, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb

2 Images
Immunocytochemistry - Alexa Fluor® 488 Anti-CD16 antibody [EPR22409-124] (AB270139)
  • ICC

Lab

Immunocytochemistry - Alexa Fluor® 488 Anti-CD16 antibody [EPR22409-124] (AB270139)

Immunofluorescence analysis of a subset of human PBMCs (human peripheral blood mononuclear cells) labeling CD16 with ab270139.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4 °C with ab270139 at 1/100 dilution (Green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (Red). Nuclear DNA was labelled with DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in cells fixed with 80% methanol (5 min).

Flow Cytometry - Alexa Fluor® 488 Anti-CD16 antibody [EPR22409-124] (AB270139)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-CD16 antibody [EPR22409-124] (AB270139)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab270139 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/mL human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab270139) or Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) isotype (100 μL at 0.2μg/mL (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD14.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live single cells.

不同偶联物与剂型 (6)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD16 antibody [EPR22409-124]

  • 578 PE

    PE Anti-CD16 antibody [EPR22409-124]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD16 antibody [EPR22409-124]

  • Unconjugated

    Anti-CD16 antibody [EPR22409-124]

  • Carrier free

    Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (Detector)

  • Carrier free

    Anti-CD16 antibody [EPR22409-124] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22409-124

亚型

IgG

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

反应种属

Human

应用

ICC, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p>This product gave a positive signal in human PMBCs fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/250", "FlowCyt-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD16 also known as FCGR3A or CD16A protein is a low-affinity receptor for the Fc region of IgG. It weighs approximately 50-70 kDa and exists on the surface of several immune cells such as natural killer (NK) cells some populations of T cells and macrophages. The expression of CD16 mediates various cellular responses including antibody-dependent cellular cytotoxicity (ADCC) where it acts by engaging with immune complexes. Researchers often use anti-CD16 antibodies in flow cytometry to analyze the presence and quantity of CD16-expressing cells.
Biological function summary

This receptor plays a significant role in immune system regulation by contributing to the activation of immune effector cells. CD16 is not part of a larger complex but it operates in concert with CD3 proteins to trigger intracellular signaling pathways upon engagement with its ligand. Its function is important for the antibody-dependent clearance of infected or malignant cells from the body representing an essential linkage between innate and adaptive immunity.

Pathways

Signaling involving CD16 is integrated into the immune response mechanisms that include the Fc gamma receptor-mediated phagocytosis pathway. This pathway involves interactions with IgG antibodies and complements the activity of other Fc gamma receptors such as CD32 and CD64. Through these interactions CD16 enhances phagocytic activity and stimulates the secretion of cytokines and chemokines important for orchestrating an effective immune response.

CD16 plays an important role in conditions like autoimmune diseases and certain cancers. Its expression and function are important in the pathophysiology of systemic lupus erythematosus where an imbalance in immune complex clearance leads to tissue damage. In oncology CD16 aids in the immune system's recognition and elimination of tumor cells working alongside proteins such as CD3 in modulating the body's anti-tumor response. Understanding CD16's involvement in these diseases helps in developing therapeutic strategies that harness the immune system's power such as monoclonal antibody therapies targeting CD16.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
See full target information FCGR3B
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搭配使用产品

Primary Antibodies

AB281764

Alexa Fluor® 555 Anti-CD16 antibody [EPR16784]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD16 antibody [EPR16784] (AB281764)

  • 0
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Secondary Antibodies

AB150077

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB197701

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (AB197701)

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Primary Antibodies

AB270112

Alexa Fluor® 488 Anti-CD80 antibody [EPR22183]

RabMAb|Recombinant

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