重组Alexa Fluor® 488荧光Anti-CD11a抗体[EPR26928-111A] (ab317289)
![Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)](https://www.abcam.cn/ps/products/317/ab317289/Images/ab317289-6-benefitsofrecombinantantibodies.jpg "Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR26928-111A] to CD11a
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 488荧光Anti-CD11a抗体[EPR26928-111A]
参阅全部 CD11a 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR26928-111A] to CD11a -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- ICC/IF: THP-1 cells. Flow Cyt: THP-1 and Human PBMC cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR26928-111A -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317289于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/500.
|
|
| ICC/IF |
1/50.
|
| 说明 |
|---|
|
Flow Cyt
1/500. |
|
ICC/IF
1/50. |
靶标
-
功能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. -
组织特异性
Leukocytes. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3683 Human
- Omim: 153370 Human
- SwissProt: P20701 Human
- Unigene: 174103 Human
-
别名
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
see all
图片
-
Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)
-
![Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)](https://www.abcam.cn/ps/products/317/ab317289/Images/ab317289-5-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289) -
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)](https://www.abcam.cn/ps/products/317/ab317289/Images/ab317289-1-alexa-fluor-488-anti-cd11a-antibody-epr26928-111a-immunocytochemistry-thp-1.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling CD11a with ab317289 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing membranous staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Negative control: HeLa (PMID: 12036881). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
-
![Flow Cytometry - Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)](https://www.abcam.cn/ps/products/317/ab317289/Images/ab317289-2-alexa-fluor-488-anti-cd11a-antibody-epr26928-111a-flow-cytometry-hela.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD11a with ab317289 at 1/500 dilution(0.1ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Negative control: HeLa (PMID:12036881). Gated on viable cells. -
![Flow Cytometry - Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)](https://www.abcam.cn/ps/products/317/ab317289/Images/ab317289-3-alexa-fluor-488-anti-cd11a-antibody-epr26928-111a-flow-cytometry-human-pbmc.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD11a with ab317289 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.
Gated on viable cells. Cells are co-stained with CD3 conjugated to Alexa Fluor® 647.
-
![Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)](https://www.abcam.cn/ps/products/317/ab317289/Images/ab317289-4-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 488 Anti-CD11a antibody [EPR26928-111A] (ab317289)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab317289 尚未被引用在任何文献中。