AB197904

Alexa Fluor® 488荧光Anti-ATPB抗体[3D5] - Mitochondrial Marker

Name: Alexa Fluor® 488 Anti-ATPB抗体[3D5] - Mitochondrial Marker (ab197904)| Abcam中文官网
Brand: Abcam Limited
SKU: AB197904
Price: 5033 CNY
Availability: InStock

Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker

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(2 Publications)

Anti-ATPB antibody [3D5] conjugated to Alexa Fluor® 488 - suitable for ICC/IF, and reacts with human samples. Clone [3D5] is a mouse monoclonal antibody cited in 11 publications.

查看别名

ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB197904)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB197904)

ab197904 staining ATPB in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197904 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min)

不同偶联物与剂型 (3)

Unconjugated

Anti-ATPB antibody [3D5] - Mitochondrial Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ATPB antibody [3D5] - Mitochondrial Marker
HRP

HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

3D5

亚型

IgG1

轻链类型

kappa

偶联物

Alexa Fluor® 488

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

ICC/IF

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.

Biological function summary

ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.

Pathways

ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.

ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalytic subunit beta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable) (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the subunit alpha (ATP5F1A), forms the catalytic core in the F(1) domain (PubMed : 37244256).

See full target information ATP5F1B

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Virulence 16:2530164 PubMed40667873

2025

Ferroptosis is important for replication and virulence and .

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Yu Li,Chun-Xue Zhou,Bing Han,Hany M Elsheikha,Hui-Jie Qiu,Xu-Dian An,Ting Zeng,Dai-Ang Liu,Qing Yang,Xing-Quan Zhu,Huai-Yu Zhou

Scientific reports 10:7795 PubMed32385361

2020

Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Liu,Miao Yu,Xuanze Shang,My Hong Hoai Nguyen,Shanmuganathan Balakrishnan,Rachel Sager,Huaiyu Hu

View all publications

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Alexa Fluor® 488荧光Anti-ATPB抗体[3D5] - Mitochondrial Marker