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重组RabMAb

重组Alexa Fluor® 488荧光Anti-Annexin A1/ANXA1抗体[EPR19342] (ab225513)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)
  • Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR19342] to Annexin A1/ANXA1
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 647 APC Carrier Free PE Unconjugated

概述

  • 产品名称

    Alexa Fluor® 488荧光Anti-Annexin A1/ANXA1抗体[EPR19342]
    参阅全部 Annexin A1/ANXA1 一抗

  • 描述

    Alexa Fluor® 488荧光兔单克隆抗体[EPR19342] to Annexin A1/ANXA1

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Mouse
    预测可用于: Rat, Human

  • 免疫原

    Recombinant fragment within Human Annexin A1/ANXA1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P04083

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • ICC/IF: NIH3T3 cells. Flow Cyt (intra): NIH3T3 cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225513于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100.

This product gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Flow Cyt (Intra)
1/5000.
说明
ICC/IF
1/100.

This product gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Flow Cyt (Intra)
1/5000.

靶标

  • 功能

    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

  • 序列相似性

    Belongs to the annexin family.
    Contains 4 annexin repeats.

  • 结构域

    A pair of annexin repeats may form one binding site for calcium and phospholipid.

  • 翻译后修饰

    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.

  • 细胞定位

    Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

图片

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)

    Overlay histogram showing NIH3T3 cells stained with ab225513 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225513, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)

    ab225513 staining Annexin A1 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225513 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in NIH3T3 cells fixed with 4% formaldehyde (10 min).

  • Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)
    Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab225513？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab225513 被引用在 1 文献中.

  • Koh JY  et al. Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4-/- mouse. BMC Med Genomics 16:133 (2023). PubMed: 37322474

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