Alexa Fluor® 488荧光Anti-alpha Tubulin抗体[DM1A] - Microtubule Marker (ab195887)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)](https://www.abcam.cn/ps/products/195/ab195887/Images/ab195887-268471-anti-alpha-tubulin-antibody-dm1a-microtubule-marker-alexa-fluor-488-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Alexa Fluor® 488荧光Anti-alpha Tubulin抗体[DM1A] - Microtubule Marker
参阅全部 alpha Tubulin 一抗 -
描述
Alexa Fluor® 488荧光小鼠单克隆抗体[DM1A] to alpha Tubulin - Microtubule Marker -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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表位
aa 426-450 -
阳性对照
- ICC/IF: HeLa cells. Flow Cyt (Intra): HeLa cells.
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常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
DM1A -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
- Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
- HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
- FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab195887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| ICC/IF | (1) |
1/150 - 1/250.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/500. |
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ICC/IF
1/150 - 1/250. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: Q5XIF6 Rat
- Unigene: 75318 Human
see all -
别名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
ab195887 staining alpha Tubulin in MEF1 cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized in 0.1% PBS-Triton X-100 for 5 minutes then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labeled in blue with DAPI.
This product gave a positive signal in 100% methanol (10 minutes) fixed MEF1 cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)](https://www.abcam.cn/ps/products/195/ab195887/Images/ab195887-256991-anti-alpha-tubulin-antibody-dm1a-microtubule-marker-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab195887 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195887, 1/500 dilution) for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 (monoclonal) Alexa Fluor® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)](https://www.abcam.cn/ps/products/195/ab195887/Images/ab195887-233424-anti-alpha-tubulin-antibody-dm1a-microtubule-marker-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)ab195887 staining alpha Tubulin in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized in 0.1% PBS-Triton X-100 for 5 minutes then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab195887 at 1/167 dilution (shown in green) overnight at +4°C. Nuclear DNA was labeled in blue with DAPI.
This product gave a positive signal in 100% methanol (10 minutes) fixed HeLa cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab195887 被引用在 11 文献中.
- Liao X et al. Significant decrease of maternal mitochondria carryover using optimized spindle-chromosomal complex transfer. PLoS Biol 21:e3002313 (2023). PubMed: 37796762
- Tammaccaro SL et al. TEAD Inhibitors Sensitize KRASG12C Inhibitors via Dual Cell Cycle Arrest in KRASG12C-Mutant NSCLC. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37111311
- Mo XL et al. Age-related SUMOylation of PLK1 is essential to meiosis progression in mouse oocytes. J Cell Physiol 237:4580-4590 (2022). PubMed: 36317691
- Castro B et al. SCA® Slows the Decline of Functional Parameters Associated with Senescence in Skin Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35742982
- Cui H et al. Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy. J Cell Mol Med 25:9634-9646 (2021). PubMed: 34486218