AB251586

重组Anti-Aldosterone抗体[EPPTX-R9-134-1] - BSA and Azide free

Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal Aldosterone antibody. Carrier free. Suitable for cELISA, ELISA and reacts with Chemical samples.

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Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)

cELISA

Unknown

Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)

This data was developed using ab241065, the same antibody clone in a different buffer formulation.

ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065) at 20 ng/ml.

ab241065 is specific to Aldosterone and the percent cross reactivity with other compounds is demonstrated in the table.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)

ELISA

Unknown

ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)

This data was developed using ab241065, the same antibody clone in a different buffer formulation.

Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Aldosterone (0, 25, 50, 100, 200, 400, 800, 1600pg/mL) was added (25μL). This was followed by adding 50μL HRP-conjugated Aldosterone and 20-25ng/mL of ab241065 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 5 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

不同偶联物与剂型 (1)

Unconjugated

Anti-Aldosterone antibody [EPPTX-R9-134-1]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPPTX-R9-134-1

亚型

IgG

不含载体蛋白

Yes

应用

cELISA, ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

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产品详情

ab251586 is the carrier-free version of ab241065.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Aldosterone sometimes called aldosteron or aldo sterone is a steroid hormone with a molecular weight of approximately 360 Da. It is synthesized and secreted by the adrenal cortex specifically in the zona glomerulosa which is the outermost layer. The production of aldosterone involves a series of enzymatic actions converting cholesterol into progesterone and eventually forming this mineralocorticoid hormone. Its expression is facilitated by the circadian rhythm and influenced by plasma potassium levels and the renin-angiotensin system.

Biological function summary

Aldosterone plays an essential role in regulating electrolyte balance and blood pressure by increasing sodium reabsorption and potassium excretion in the kidney's distal tubules and collecting ducts. As part of a system with other hormones like cortisol and aldosterone it modulates the body's fluid and electrolyte equilibrium. Through binding and activating the mineralocorticoid receptor aldosterone adjusts ion transport in epithelial and non-epithelial tissues impacting processes like fluid retention and blood pressure maintenance.

Pathways

Aldosterone significantly influences the renin-angiotensin-aldosterone system (RAAS) and the sodium-potassium pump. The RAAS is critical in maintaining blood pressure and fluid balance where aldosterone works closely with angiotensin II and renin. Within this pathway aldosterone promotes sodium reabsorption leading to water retention and its action is closely tied to the function of the sodium-potassium pump which further regulates cellular ionic balance and impacts systemic vascular resistance.

Aldosterone is associated with conditions such as hyperaldosteronism and hypertension. In hyperaldosteronism excessive aldosterone levels cause high blood pressure and hypokalemia due to increased sodium and water retention. This condition often results from adrenal gland abnormalities that affect aldosterone production. Proteins related to this disorder through the RAAS include angiotensin-converting enzyme and angiotensinogen highlighting the interplay between aldosterone and these components. Additionally aldosterone has links to congestive heart failure where its overactivity can exacerbate fluid overload worsening cardiac function.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Aldosterone

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重组Anti-Aldosterone抗体[EPPTX-R9-134-1] - BSA and Azide free