Anti-Aldolase B抗体[5E2AD2] (ab129728)
Key features and details
- Mouse monoclonal [5E2AD2] to Aldolase B
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Aldolase B抗体[5E2AD2]
参阅全部 Aldolase B 一抗 -
描述
小鼠单克隆抗体[5E2AD2] to Aldolase B -
宿主
Mouse -
经测试应用
适用于: IP, ICC/IF, Flow Cytmore details
不适用于: WB -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- Human, Rat and Mouse liver homogenate lysates; HepG2 cells; Hela cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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纯化说明
Purity is near homogeneity as judged by SDS-PAGE. ab129728 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
5E2AD2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129728于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use a concentration of 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use a concentration of 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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通路
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
疾病相关
Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. -
序列相似性
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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数据库链接
- Entrez Gene: 229 Human
- Entrez Gene: 230163 Mouse
- Entrez Gene: 24190 Rat
- Omim: 612724 Human
- SwissProt: P05062 Human
- SwissProt: Q91Y97 Mouse
- SwissProt: P00884 Rat
- Unigene: 530274 Human
see all -
别名
- ALDB antibody
- ALDO B antibody
- ALDO2 antibody
see all
图片
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Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1: ladder Lane2: rat brain homogenate lysate Lane3: rat liver homogenate lysate Lane4: rat skeletal muscle homogenate lysate
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Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.
Lane 1: Ladder Lane 2: Human liver homogenate lysate
Lane 3: Rat liver homogenate lysate Lane 4: Mouse liver homogenate lysate -
Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm.
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Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab129728 被引用在 1 文献中.
- Ming G et al. Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis. Transl Pediatr 10:2083-2094 (2021). PubMed: 34584879