AB129728

Anti-Aldolase B抗体[5E2AD2]

Name: Aldolase B抗体[5E2AD2] (ab129728)| Abcam中文官网
Brand: Abcam Limited
SKU: AB129728
Price: 4519 CNY
Availability: InStock

Anti-Aldolase B antibody [5E2AD2]

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(2 Publications)

Mouse Monoclonal Aldolase B antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

查看别名

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

4 Images

Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Flow Cyt

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Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (AB129728)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm.

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Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.

Lane 1 : Ladder
Lane 2 : Human liver homogenate lysate
Lane 3 : Rat liver homogenate lysate
Lane 4 : Mouse liver homogenate lysate

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Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)

Predicted band size: 39 kDa

false

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Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1 : ladder Lane2 : rat brain homogenate lysate Lane3 : rat liver homogenate lysate Lane4 : rat skeletal muscle homogenate lysate

All lanes:

Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)

Predicted band size: 39 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

5E2AD2

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, Flow Cyt, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化说明

Purity is near homogeneity as judged by SDS-PAGE. ab129728 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

存储溶液

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase B also known as fructose-bisphosphate aldolase B is an enzyme critical in fructose metabolism. It facilitates the cleavage of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate both vital intermediates in glycolysis and gluconeogenesis. Aldolase B has a molecular mass of approximately 39 kDa. It is expressed mainly in the liver with lower levels found in the kidneys and intestines.

Biological function summary

This enzyme plays an important role in the breakdown of dietary sugars. The primary biological role of aldolase B is its function in the liver-specific pathway of fructose catabolism. It does not function as part of a large multiprotein complex but operates as a homotetramer. Aldolase B's activity helps to regulate blood sugar levels and manage energy sources especially when glucose is not readily available.

Pathways

Aldolase B is involved in the gluconeogenesis and glycolysis pathways. In gluconeogenesis it helps in the formation of glucose from non-carbohydrate sources which is important during fasting states. In glycolysis it aids in the breakdown of glucose to produce energy. This enzyme therefore interacts with other glycolytic enzymes such as phosphofructokinase and pyruvate kinase which are part of the same metabolic pathways.

Defects in aldolase B activity can lead to hereditary fructose intolerance a potentially serious condition caused by the inability to properly metabolize fructose. This disorder often results in symptoms such as hypoglycemia and jaundice following fructose ingestion. The disorder can also relate to liver and kidney dysfunctions as these organs express aldolase B most prominently. Deficient aldolase B function disrupts the metabolic pathways where the enzyme interacts with other important proteins and enzymes involved in energy balance.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed : 10970798, PubMed : 12205126, PubMed : 20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed : 35122041).

See full target information ALDOB

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Translational pediatrics 10:2083-2094 PubMed34584879

2021

Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Gedong Ming,Wanliang Guo,Yuan Cheng,Jian Wang

PloS one 12:e0170575 PubMed28129380

2017

The Identification and Distribution of Cattle XCR1 and XCL1 among Peripheral Blood Cells: New Insights into the Design of Dendritic Cells Targeted Veterinary Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Guoyan Wei,Yimei Cao,Dong Li,Pinghua Li,Jing Zhang,Huifang Bao,Yingli Chen,Yuanfang Fu,Pu Sun,Xingwen Bai,Xueqing Ma,Zengjun Lu,Zaixin Liu

View all publications

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Anti-Aldolase B抗体[5E2AD2]

Anti-Aldolase B antibody [5E2AD2]

Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (AB129728)

Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (AB129728)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原