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重组RabMAb

重组Anti-Aldolase B + Aldolase C抗体[EPR3138Y] - BSA and Azide free (ab223527)

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Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
  • Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC HRP PE Unconjugated

概述

  • 产品名称

    Anti-Aldolase B + Aldolase C抗体[EPR3138Y] - BSA and Azide free

  • 描述

    兔单克隆抗体[EPR3138Y] to Aldolase B + Aldolase C - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WB, Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC: Human, mouse and rat kidney tissues. WB: Human, mouse and rat kidney lysate. His-tagged human Aldolase B and C full-length recombinant protein.

  • 常规说明

    ab223527 is the carrier-free version of ab75751.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223527于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

图片

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution

    Lane 1 : His-tagged human Aldolase A full-length recombinant protein
    Lane 2 : His-tagged human Aldolase B full-length recombinant protein
    Lane 3 : His-tagged human Aldolase C full-length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa


    Exposure time: 40 seconds


    This data was developed using ab75751, the same antibody clone in a different buffer formulation. 

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution (Purified)

    Lane 1 : Human kidney lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    This data was developed using ab75751, the same antibody clone in a different buffer formulation. 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

    This data was developed using ab75751, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab223527 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

    This data was developed using ab75751, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab223527 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

    This data was developed using ab75751, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab223527 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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