重组Anti-ALDH7A1抗体[EP1935Y] (ab53278)

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling ALDH7A1 with ab53278 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab53278 overnight at 4° C. Nucleus were visualized using DAPI.

HepG2 cells were fixed with 100μl 4% PFA for 10 min at RT, then permeabilized with 100μl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab53278 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.

ALDH7A1 was immunoprecipitated from 0.35 mg of human liver tissue lysate with the antibody at 1/20 dilution. Western blot was performed from the immunoprecipitated using antibody at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used as secondary antibody at 1/5000 dilution.

Lane 1: Human liver tissue lysate 10 μg.
Lane 2: Human liver tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab53278 in human liver tissue lysate.