重组Anti-ALDH5A1/SSADH抗体[EPR7794] (ab129017)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7794] to ALDH5A1/SSADH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-ALDH5A1/SSADH抗体[EPR7794]
参阅全部 ALDH5A1/SSADH 一抗 -
描述
兔单克隆抗体[EPR7794] to ALDH5A1/SSADH -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human ALDH5A1/SSADH aa 500-600. The exact sequence is proprietary.
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阳性对照
- Fetal liver and A431 lysates Human liver tissue Mouse kidney tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.16 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7794 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129017于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). -
组织特异性
Brain, pancreas, heart, liver, skeletal muscle and kidney. Lower in placenta. -
通路
Amino-acid degradation; 4-aminobutanoate degradation. -
疾病相关
Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development. -
序列相似性
Belongs to the aldehyde dehydrogenase family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 7915 Human
- Entrez Gene: 214579 Mouse
- Entrez Gene: 291133 Rat
- Omim: 610045 Human
- SwissProt: P51649 Human
- SwissProt: Q8BWF0 Mouse
- SwissProt: P51650 Rat
- Unigene: 371723 Human
see all -
别名
- Aldedehyde dehydrogenase 5 family antibody
- Aldehyde dehydrogenase 5 family member A1 antibody
- Aldehyde dehydrogenase 5A1 antibody
see all
图片
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All lanes : Anti-ALDH5A1/SSADH antibody [EPR7794] (ab129017) at 1/10000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : A431 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody [EPR7794] (ab129017)
ab129017, at 1/100 dilution staining ALDH5A1/SSADH in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody [EPR7794] (ab129017)
ab129017, at 1/100 dilution staining ALDH5A1/SSADH in formalin fixed paraffin embedded Mouse kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab129017 被引用在 4 文献中.
- Brennenstuhl H et al. Succinic Semialdehyde Dehydrogenase Deficiency: In Vitro and In Silico Characterization of a Novel Pathogenic Missense Variant and Analysis of the Mutational Spectrum of ALDH5A1. Int J Mol Sci 21:N/A (2020). PubMed: 33203024
- Zheng J et al. Interneuron Accumulation of Phosphorylated tau Impairs Adult Hippocampal Neurogenesis by Suppressing GABAergic Transmission. Cell Stem Cell 26:331-345.e6 (2020). PubMed: 31978364
- Hujber Z et al. GABA, glutamine, glutamate oxidation and succinic semialdehyde dehydrogenase expression in human gliomas. J Exp Clin Cancer Res 37:271 (2018). PubMed: 30404651
- Shim BY et al. Glucose transporter 1 (GLUT1) of anaerobic glycolysis as predictive and prognostic values in neoadjuvant chemoradiotherapy and laparoscopic surgery for locally advanced rectal cancer. Int J Colorectal Dis 28:375-83 (2013). PubMed: 22847606