重组Anti-ALDH2抗体[EPR4494] (ab133306)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4494] to ALDH2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ALDH2抗体[EPR4494]
参阅全部 ALDH2 一抗 -
描述
兔单克隆抗体[EPR4494] to ALDH2 -
宿主
Rabbit -
特异性
The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely. -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SW480 and HepG2 cell lysates; Human heart, fetal liver, mouse liver, rat liver, and mouse lung lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4494 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
靶标
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通路
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
序列相似性
Belongs to the aldehyde dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P05091 Human
- SwissProt: P47738 Mouse
- SwissProt: P11884 Rat
- Unigene: 604551 Human
see all -
别名
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
图片
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All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution
Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : ALDH2 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab133306 observed at 53 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot. Loss of signal was observed when ALDH2 knockout sample was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution
Lane 1 : HepG2 lysates
Lane 2 : Human heart lysates
Lane 3 : Fetal liver lysates
Lane 4 : Mouse liver lysates
Lane 5 : Rat liver lysates
Lane 6 : Mouse lung lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133306 被引用在 2 文献中.
- Ding H et al. Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells. Korean J Physiol Pharmacol 26:183-193 (2022). PubMed: 35477546
- Bardallo RG et al. Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach. Int J Mol Sci 22:N/A (2021). PubMed: 34069402