Anti-ALDH2抗体[9A10AF10] (ab110311)
![Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10] (ab110311)](https://www.abcam.cn/ps/products/110/ab110311/Images/ab110311-123587-anti-aldh2-antibody-9a10af10-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10] (ab110311)")
Key features and details
- Mouse monoclonal [9A10AF10] to ALDH2
- Suitable for: ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-ALDH2抗体[9A10AF10]
参阅全部 ALDH2 一抗 -
描述
小鼠单克隆抗体[9A10AF10] to ALDH2 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- HepG2 cells; Human, Rat and Mouse liver and HepG2 whole cell lysate (ab7900).
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯化说明
ab110311 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
9A10AF10 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml. For 2 hours.
|
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| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. For 2 hours. |
|
IP
Use at an assay dependent concentration. |
靶标
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通路
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
序列相似性
Belongs to the aldehyde dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P05091 Human
- SwissProt: P47738 Mouse
- SwissProt: P11884 Rat
- Unigene: 604551 Human
see all -
别名
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10] (ab110311)Immunocytochemistry analysis using ab110311 at 5µg/ml staining ALDH2 in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: Target protein locates mainly in the mitochondrial matrix. -
![Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10] (ab110311)](https://www.abcam.cn/ps/products/110/ab110311/Images/ab110311-123586-anti-aldh2-antibody-9a10af10-immunoprecipitation.gif)
Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10] (ab110311)Using MitoSciences' standard immunoprecipitation protocol, ab110311 precipitates down the 54kDa mature ALDH2 protein from human, mouse, and rat liver tissue lysate and HepG2 culture cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab110311 尚未被引用在任何文献中。