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Anti-ALDH1A3抗体[OTI4E8] - BSA and Azide free (ab273651)

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Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
  • Flow Cytometry (Intracellular) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

Key features and details

  • Mouse monoclonal [OTI4E8] to ALDH1A3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-ALDH1A3抗体[OTI4E8] - BSA and Azide free
    参阅全部 ALDH1A3 一抗

  • 描述

    小鼠单克隆抗体[OTI4E8] to ALDH1A3 - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Recombinant fragment corresponding to Human ALDH1A3. Amino acids 1-100 and 413-512.
    Database link: P47895

  • 阳性对照

    • WB: Transfected HEK-293T, A459 cell lysate; IHC-P: Human prostate and prostate carcinoma tissue; Flow Cyt (Intra): Jurkat whole cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273651于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.
WB
1/200 - 1/2000. Predicted molecular weight: 56 kDa.
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
1/100.
WB
1/200 - 1/2000. Predicted molecular weight: 56 kDa.
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    All lanes : Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651) at 1/500 dilution

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY ALDH1A3-transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 56 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

    Immunohistochemical analysis of paraffin-embedded human prostate tissue staining ALDH1A3 with a mouse monoclonal ALDH1A3 antibody [OTI4E8].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Flow Cytometry (Intracellular) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Flow Cytometry (Intracellular) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

    Flow cytometric analysis of Jurkat cells using a mouse monoclonal ALDH1A3 antibody [OTI4E8] (Red), compared to a nonspecific negative control antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    All lanes : Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651) at 1/200 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : SV-T2 (Simian Virus 40-transformed BALB/c mouse embryo fibroblasts cell line) whole cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 7 : MDCK (canine kidney cell line) whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 56 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue staining ALDH1A3 with a mouse monoclonal ALDH1A3 antibody [OTI4E8].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    All lanes : Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651) at 1/200 dilution

    Lane 1 : Negative control E. coli lysate
    Lane 2 : E. coli lysate containing human ALDH1A3 recombinant protein fragment gene

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 56 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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