Anti-ALDH1A1抗体(ab131068)
Key features and details
- Rabbit polyclonal to ALDH1A1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-ALDH1A1抗体
参阅全部 ALDH1A1 一抗 -
描述
兔多克隆抗体to ALDH1A1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to internal sequence amino acids of Human ALDH1A1.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131068于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 54 kDa.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 54 kDa. |
靶标
-
功能
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
通路
Cofactor metabolism; retinol metabolism. -
序列相似性
Belongs to the aldehyde dehydrogenase family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
- SwissProt: P51647 Rat
- Unigene: 76392 Human
see all -
别名
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
图片
-
Western blot - Anti-ALDH1A1 antibody (ab131068)All lanes : Anti-ALDH1A1 antibody (ab131068) at 0.5 µg/ml
Lane 1 : Human A549 whole cell lysates
Lane 2 : Human HepG2 whole cell lysates
Lane 3 : Rat liver tissue lysates
Lane 4 : Rat kidney tissue lysates
Lane 5 : Mouse liver tissue lysates
Lane 6 : Mouse kidney tissue lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking buffer: 5% non-fat milk/TBS.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab131068)Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling ALDH1A1 with ab131068 at 2 μg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using a HRP Conjugated Rabbit IgG assay kit with DAB as the chromogen.
数据表及文件
-
Datasheet download
文献 (1)
ab131068 被引用在 1 文献中.
- Gupta V et al. Expression of aldehyde dehydrogenase 1A1 in oral squamous cell carcinoma and its correlation with clinicopathological parameters. Natl J Maxillofac Surg 13:208-215 (2022). PubMed: 36051794
