Anti-ALDH1A1抗体[5A11] (ab105920)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)](https://www.abcam.cn/ps/products/105/ab105920/Images/ab105920-118296-anti-aldh1a1-antibody-5a11-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)")
Key features and details
- Mouse monoclonal [5A11] to ALDH1A1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ALDH1A1抗体[5A11]
参阅全部 ALDH1A1 一抗 -
描述
小鼠单克隆抗体[5A11] to ALDH1A1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Purified recombinant fragment of Human ALDH1A1 expressed in E. coli.
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阳性对照
- WB: Raji, Jurkat, THP1 and K562 cell lysates IHC-P: Human cerebellum and lung cancer tissues
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR120645-17 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
5A11 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105920于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/200 - 1/1000. Predicted molecular weight: 55 kDa.
|
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| IHC-P |
1/200 - 1/1000.
|
|
| Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/200 - 1/1000. Predicted molecular weight: 55 kDa. |
|
IHC-P
1/200 - 1/1000. |
|
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
通路
Cofactor metabolism; retinol metabolism. -
序列相似性
Belongs to the aldehyde dehydrogenase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 216 Human
- Omim: 100640 Human
- SwissProt: P00352 Human
- Unigene: 76392 Human
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别名
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human cerebellum tissue by Immunohistochemistry.
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![Flow Cytometry - Anti-ALDH1A1 antibody [5A11] (ab105920)](https://www.abcam.cn/ps/products/105/ab105920/Images/ab105920-195308-anti-aldh1a1-antibody-5a11-flow-cytometry.jpg)
Flow Cytometry - Anti-ALDH1A1 antibody [5A11] (ab105920)Overlay histogram showing HepG2 cells stained with ab105920 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105920, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-ALDH1A1 antibody [5A11] (ab105920)](https://www.abcam.cn/ps/products/105/ab105920/Images/ab105920-118297-anti-aldh1a1-antibody-5a11-western-blot.jpg)
Western blot - Anti-ALDH1A1 antibody [5A11] (ab105920)All lanes : Anti-ALDH1A1 antibody [5A11] (ab105920) at 1/200 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : K562 cell lysate
Predicted band size: 55 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)](https://www.abcam.cn/ps/products/105/ab105920/Images/ab105920-118295-anti-aldh1a1-antibody-5a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human lung cancer tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab105920 被引用在 6 文献中.
- Fahmi MN et al. High ALDH-1 Expression Predicts Non-Complete Response of Radiotherapy in Stage III Squamous Cell Cervical Carcinoma Patients. Asian Pac J Cancer Prev 24:1863-1868 (2023). PubMed: 37378913
- Gunduz M et al. Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer. Oncol Lett 19:965-971 (2020). PubMed: 31897209
- Sulaiman A et al. Dual inhibition of Wnt and Yes-associated protein signaling retards the growth of triple-negative breast cancer in both mesenchymal and epithelial states. Mol Oncol N/A:N/A (2018). WB . PubMed: 29316250
- Jia D et al. An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells. Cell Death Dis 8:e2932 (2017). PubMed: 28703802
- Jia D et al. Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo. Oncotarget 7:771-85 (2016). WB . PubMed: 26506421
- Martinez-Cruzado L et al. Aldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations. Sci Rep 6:27878 (2016). WB, IF . PubMed: 27292183