AB248799

重组Anti-ALDH16A1抗体[EPR9216] - BSA and Azide free

Anti-ALDH16A1 antibody [EPR9216] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal A16A1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

查看别名

Aldehyde dehydrogenase family 16 member A1, ALDH16A1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ALDH16A1 antibody [EPR9216] - BSA and Azide free (AB248799)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ALDH16A1 antibody [EPR9216] - BSA and Azide free (AB248799)

This data was developed using ab137073, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling ALDH16A1 with ab137073 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH16A1 antibody [EPR9216] - BSA and Azide free (AB248799)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH16A1 antibody [EPR9216] - BSA and Azide free (AB248799)

This data was developed using ab137073, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling ALDH16A1 with ab137073 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH16A1 antibody [EPR9216] - BSA and Azide free (AB248799)

This data was developed using ab137073, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ALDH16A1 with ab137073 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ALDH16A1 antibody [EPR9216] - BSA and Azide free (AB248799)

This data was developed using ab137073, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ALDH16A1 antibody [EPR9216] (<a href='/products/primary-antibodies/aldh16a1-antibody-epr9216-ab137073'>ab137073</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-ALDH16A1 antibody [EPR9216]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9216

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab248799 is the carrier-free version of ab137073.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ALDH16A1 or aldehyde dehydrogenase 16 family member A1 belongs to the aldehyde dehydrogenase superfamily. This protein does not have well-documented enzymatic activity but it shares structural similarity with other family proteins suggesting a potential role in aldehyde metabolism. ALDH16A1 has a molecular mass of approximately 83 kDa. The protein expresses mainly in liver kidney and other tissues though its exact function remains unclear.

Biological function summary

Members of the aldehyde dehydrogenase family act in detoxification processes by oxidizing aldehydes into carboxylic acids reducing cellular damage. While ALDH16A1's activity remains undetermined its expression profile implies involvement in pathways where aldehyde metabolism impacts critical cellular functions. It does not form a known complex but its similarity with other aldehyde dehydrogenases suggests a supporting role in cellular detoxification pathways.

Pathways

Amino acid metabolism and detoxification pathways potentially involve ALDH16A1 due to its homology with other aldehyde dehydrogenases. This protein potentially interacts with enzymes such as ALDH2 which participates in ethanol metabolism and detoxification. Both proteins play roles in mitigating oxidative stress and processing toxic aldehydes ensuring cellular homeostasis.

Polymorphisms or dysregulation in ALDH16A1 may correlate with metabolic disorders particularly those affecting kidney function. Further research implicates altered expression of this protein in conditions like diabetic nephropathy. The association with diabetes might tie into dysregulated aldehyde detoxification linking ALDH16A1 indirectly to proteins like ALDH2 known for roles in managing reactive aldehyde species and oxidative stress.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information ALDH16A1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn

View all publications

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重组Anti-ALDH16A1抗体[EPR9216] - BSA and Azide free